| 长期施肥对不同深度稻田土壤碳氮水解酶活性的影响特征 |
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| 引用本文： | 杜林森,唐美铃,祝贞科,魏亮,魏晓梦,周萍,葛体达,王久荣,邹冬生,吴金水.长期施肥对不同深度稻田土壤碳氮水解酶活性的影响特征[J].环境科学,2018,39(8):3901-3909. |
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| 作者姓名： | 杜林森 唐美铃 祝贞科 魏亮 魏晓梦 周萍 葛体达 王久荣 邹冬生 吴金水 |
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| 作者单位： | 湖南农业大学生物科学技术学院;中国科学院亚热带农业生态研究所亚热带农业生态过程重点实验室;中南林业科技大学生命科学与技术学院;湖南省农业科学院农业部长江中游平原农业环境重点实验室 |
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| 基金项目： | 国家重点研发计划项目（2016YFD0300902）；国家自然科学基金项目（41501321）；湖南省自然科学基金项目（2016JJ3132，2016JJ2136）；农业部长江中游平原农业环境重点实验室开放基金项目 |
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| 摘 要： | 与稻田土壤碳周转密切相关的酶活性是评价土壤肥力和肥料管理的重要指标.本研究选取秸秆还田(ST)、化肥(NPK)和不施肥(CK)的长期定位试验田,以10 cm的间距分段采集土壤剖面0~40 cm范围内的新鲜土样,利用96微孔酶标板荧光分析法,测定参与土壤碳氮转化过程关键酶β-1,4-葡萄糖苷酶(BG)和β-1,4-N-乙酰氨基葡萄糖苷酶(NAG)活性,探讨不同施肥措施对不同深层土壤酶活的影响.结果表明,相比不施肥的土壤,施用化肥和秸秆土壤的BG酶活性分别提高了35%~118%和55%~342%;NAG酶活性分别提高了9%~30%和102%~484%.同时,随着土层深度的增加,土壤酶活性逐渐降低,0~20 cm深层土壤酶活性显著高于20~40 cm深层土壤.在不同施肥措施中秸秆还田可高程度影响稻田深层土壤.RDA分析表明土壤碳氮含量主要与0~20 cm的土壤酶活性有显著的正相关关系,与20~40 cm的土壤酶活性呈负相关关系.综上所述,随着土壤深度增加土壤微生物量和土壤酶活性显著降低.长期施肥显著提高了不同深层土壤生物量和土壤酶活性,其中秸秆还田作用尤为突出.因此,合理的秸杆还田有利于改善稻田深层土壤肥力,优化农田土壤养分循环,为作物生长提供良好的土壤环境.
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| 关 键 词： | 水稻土 长期施肥 土层 酶活性 碳氮水解酶 米氏方程 |
| 收稿时间： | 2017/11/25 0:00:00 |
| 修稿时间： | 2018/2/24 0:00:00 |
Effects of Long-term Fertilization on Enzyme Activities in Profile of Paddy Soil Profiles |
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| DU Lin-sen,TANG Mei-ling,ZHU Zhen-ke,WEI Liang,WEI Xiao-meng,ZHOU Ping,GE Ti-d,WANG Jiu-rong,ZOU Dong-sheng and WU Jin-shui.Effects of Long-term Fertilization on Enzyme Activities in Profile of Paddy Soil Profiles[J].Chinese Journal of Environmental Science,2018,39(8):3901-3909. |
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| Authors: | DU Lin-sen TANG Mei-ling ZHU Zhen-ke WEI Liang WEI Xiao-meng ZHOU Ping GE Ti-d WANG Jiu-rong ZOU Dong-sheng and WU Jin-shui |
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| Institution: | College of Bioscience and Biotechnology, Hunan Agriculture University, Changsha 410128, China;Key Laboratory of Subtropical Agriculture Ecology, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China,Key Laboratory of Subtropical Agriculture Ecology, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;College of Life Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China,Key Laboratory of Subtropical Agriculture Ecology, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;Key Laboratory of Agricultural Environment in the Middle Reaches of the Yangtze River, Ministry of Agriculture, Hunan Academy of Agricultural Sciences, Changsha 410125, China,Key Laboratory of Subtropical Agriculture Ecology, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China,Key Laboratory of Subtropical Agriculture Ecology, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China,Key Laboratory of Subtropical Agriculture Ecology, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China,Key Laboratory of Subtropical Agriculture Ecology, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China,Key Laboratory of Subtropical Agriculture Ecology, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China,College of Bioscience and Biotechnology, Hunan Agriculture University, Changsha 410128, China and Key Laboratory of Subtropical Agriculture Ecology, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China |
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| Abstract: | |
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| Keywords: | paddy soil long-term fertilization soil profile enzyme activity carbon and nitrogen hydrolytic enzyme Michaelis-Menten equation |
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