| 利用GC/MS代谢组学技术检测纳米TiO2对多头绒泡菌代谢途径的扰动作用 |
| |
| 引用本文： | 张天任,闵武霞,夏沁怡,李佳琦,易思卓,张建华,刘士德.利用GC/MS代谢组学技术检测纳米TiO2对多头绒泡菌代谢途径的扰动作用[J].环境科学学报,2018,38(9):3762-3769. |
| |
| 作者姓名： | 张天任 闵武霞 夏沁怡 李佳琦 易思卓 张建华 刘士德 |
| |
| 作者单位： | 深圳大学生命与海洋科学学院深圳市微生物基因工程重点实验室 |
| |
| 基金项目： | 深圳市科技研发资金基础研究计划（No.JCYJ20170302144210545，JCYJ20140418193546107，JC201105130450A）；中国博士后科学基金（No.2017M622755）；国家自然科学基金（No.31070043） |
| |
| 摘 要： | 纳米TiO_2(n TiO_2)具有广泛的应用价值.但n TiO_2在阳光或紫外线下具有很强的氧化作用,可对细胞产生强烈的毒害作用,因此其对生态环境的可能破坏作用值得关注.有关n TiO_2在暗环境中对生物的毒性作用报道还不多,还不能全面揭示n TiO_2的毒性作用机理.代谢组学分析是发现代谢差异、识别被干扰的代谢途径、了解n TiO_2毒性作用机理、评估n TiO_2细胞毒性的重要手段.本文采用GC/MS(气相色谱/质谱)技术检测了n TiO_2在暗环境下对多头绒泡菌原质团(单细胞)代谢组的影响,根据多变量识别模式分析发现了60余个被n TiO_2显著干扰的代谢物.这些代谢物涉及糖代谢、氨基酸代谢、核苷酸代谢、多胺生物合成、次生代谢途径等,为全面了解n TiO_2对细胞的毒性作用机理提供了有益补充.
|
| 关 键 词： | 纳米TiO2 多头绒泡菌 代谢组学 细胞毒性 |
| 收稿时间： | 2018/2/11 0:00:00 |
| 修稿时间： | 2018/5/31 0:00:00 |
The detection of nano-sized TiO2 induced metabolic perturbations in Physarum polycephalum with metabolomics |
| |
| ZHANG Tianren,MIN Wuxi,XIA Qinyi,LI Jiaqi,YI Sizhuo,ZHANG Jianhua and LIU Shide.The detection of nano-sized TiO2 induced metabolic perturbations in Physarum polycephalum with metabolomics[J].Acta Scientiae Circumstantiae,2018,38(9):3762-3769. |
| |
| Authors: | ZHANG Tianren MIN Wuxi XIA Qinyi LI Jiaqi YI Sizhuo ZHANG Jianhua and LIU Shide |
| |
| Institution: | College of Life Sciences and Oceanography, Shenzhen University, Shenzhen Key Laboratory of Microbial Genetic Engineering, Shenzhen 518060,College of Life Sciences and Oceanography, Shenzhen University, Shenzhen Key Laboratory of Microbial Genetic Engineering, Shenzhen 518060,College of Life Sciences and Oceanography, Shenzhen University, Shenzhen Key Laboratory of Microbial Genetic Engineering, Shenzhen 518060,College of Life Sciences and Oceanography, Shenzhen University, Shenzhen Key Laboratory of Microbial Genetic Engineering, Shenzhen 518060,College of Life Sciences and Oceanography, Shenzhen University, Shenzhen Key Laboratory of Microbial Genetic Engineering, Shenzhen 518060,College of Life Sciences and Oceanography, Shenzhen University, Shenzhen Key Laboratory of Microbial Genetic Engineering, Shenzhen 518060 and College of Life Sciences and Oceanography, Shenzhen University, Shenzhen Key Laboratory of Microbial Genetic Engineering, Shenzhen 518060 |
| |
| Abstract: | Although widely utilized, Nano-sized TiO2 (nTiO2) has a strong oxidation effect under solar or UV irradiation, which is toxic to cells and its effect on the ecological environment is worthy of attention. There are few reports about the toxic effects of nTiO2 on organisms under the dark environment and the toxic mechanism of nTiO2 is not fully understood. Metabolomic analysis is an important to discover metabolic differences, identify disturbed metabolic pathways, understand the mechanism of toxicity of nTiO2 and assess cytotoxicity of nTiO2. In the present study, gas chromatography mass spectrometry (GC/MS)-based metabolomics was used to investigate the effect of nTiO2 on cells (Physarum polycephalum) under dark conditions. According to the multivariate pattern recognition analysis, more than 60 metabolites significantly interfered by nTiO2 were found. These metabolites involved in glycometabolism, amino acid metabolism, nucleotide metabolism, polyamine biosynthesis and secondary metabolic pathways, etc, which provided a useful supplement for a comprehensive understanding of the mechanism of toxicity of nTiO2 on cells. |
| |
| Keywords: | nano-sized titanium dioxide Physarum polycephalum metabonomics cytotoxicity |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《环境科学学报》浏览原始摘要信息 |
| 点击此处可从《环境科学学报》下载免费的PDF全文 |
|
