| 偏肿拟栓菌共代谢降解芘条件的优化 |
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| 引用本文： | 温继伟,高大文.偏肿拟栓菌共代谢降解芘条件的优化[J].中国环境科学,2011,31(9):1516-1522. |
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| 作者姓名： | 温继伟 高大文 |
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| 作者单位： | 1. 东北林业大学林学院,黑龙江哈尔滨,150040
2. 东北林业大学林学院,黑龙江哈尔滨150040;哈尔滨工业大学,城市水资源与水环境国家重点实验室,黑龙江哈尔滨150090 |
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| 基金项目： | 全国优秀博士学位论文作者专项研究基金(200544); 黑龙江省杰出青年研究基金(JC200909) |
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| 摘 要： | 为提高中国东北土著白腐真菌偏肿拟栓菌(Pseudotrametes gibbosa)对高分子量多环芳烃芘的共代谢降解效果,通过正交试验研究了共代谢基质、接种量、装液量及2,2￠-连氮-二(3-乙基苯并噻唑-6-磺酸)(ABTS)的最佳配比,同时采用浓度梯度法考察芘的初始浓度对偏肿拟栓菌共代谢降解芘的影响.结果表明,偏肿拟栓菌Pseudotrametes gibbosa共代谢降解芘的最佳培养基为:麸皮浓度为20g/L,接种量为3片直径为10mm的菌片,装液量为50mL,不加ABTS,在该培养条件下,酶活可达53.41U/mL,对芘的降解率达到88.8%.初始浓度小于10mg/L的芘对菌体产酶有一定的促进作用,大于10mg/L时抑制漆酶的分泌,同时菌体对初始浓度小于90mg/L芘的降解率在22.24%~93.54%之间.
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| 关 键 词： | 共代谢 漆酶 偏肿拟栓菌 白腐真菌 芘 |
| 收稿时间： | 2011-01-19; |
Optimization of culture condition for pyrene co-metabolism by Pseudotrametes gibbosa |
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| WEN Ji-wei,GAO Da-wen.Optimization of culture condition for pyrene co-metabolism by Pseudotrametes gibbosa[J].China Environmental Science,2011,31(9):1516-1522. |
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| Authors: | WEN Ji-wei GAO Da-wen |
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| Institution: | WEN Ji-wei1,GAO Da-wen1,2(1.School of Forestry,Northeast Forestry University,Harbin 150040,China,2.State Key Laboratory of Urban Water Resource and Environment,Harbin Institute of Technology,Harbin 150090,China) |
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| Abstract: | To enhance the degradation efficiency of pyrene co-metabolism by indigenous white-rot fungus Pseudotrametes gibbosa from northeast forest area in China,orthogonal experiments were designed to get the optimum conditions for pyrene co-metabolism,and the effects of different pyrene concentrations on the degradation efficiency of pyrene co-metabolism were also investigated.The optimum conditions were as following: wheat bran with the concentration of 20g/L,three agar plugs(10mm diameter) and 50mL culture in 150... |
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| Keywords: | co-metabolism laccase Pseudotrametes gibbosa white rot fungus pyrene |
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