Effects of different pretreatment methods on anaerobic mixed microflora for hydrogen production and COD reduction from palm oil mill effluent |
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Authors: | Parviz MohammadiShaliza Ibrahim Mohamad Suffian Mohamad Annuar Sean Law |
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Institution: | a Department of Civil Engineering, Faculty of Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia b Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia |
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Abstract: | Effects of different pretreatment methods on sludge inocula were evaluated with respect to hydrogen (H2) production enhancement using palm oil mill effluent (POME) in a batch system. The experimental results showed that the pretreatment methods (chemical, acid, heat-shock, freezing and thawing, and base) at 35 °C and initial pH 5.5 had a positive influence on H2 production yield and chemical oxygen demand (COD) removal efficiency during the fermentative H2 production as compared to the control experiments (without pretreatment). Heat-shock pretreatment method was shown to be a simple and useful method for enhancing both H2 producing and COD removal processes from POME with highest H2 yield and COD removal efficiency at 0.41 mmol H2/g COD and 86%, respectively. |
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Keywords: | Enrichment Hydrogen production Pretreatment methods Inoculum POME |
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