| DHA高产菌Schizochytrium sp.FJU-512的分离及其18S rRNA基因序列比较分析 |
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| 引用本文： | 黄建忠,江贤章.DHA高产菌Schizochytrium sp.FJU-512的分离及其18S rRNA基因序列比较分析[J].应用与环境生物学报,2005,11(2):202-207. |
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| 作者姓名： | 黄建忠 江贤章 |
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| 作者单位： | 福建师范大学生物工程学院,福州,350007 |
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| 基金项目： | 国家自然科学基金项目 (No. 30370028 )，福建省科技厅重大项目(2003F005)~~ |
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| 摘 要： | 采用松花粉垂钓法分离到一株Docosahexaenoicacid(DHA)高产菌FJU 512.该菌株DHA含量高(占总脂肪酸的56. 24 % ),其它长链杂酸含量少(仅有docosapentaenoicacid, DPA),极具开发应用价值.高密度培养可获得33gL-1生物量.该菌株行二分裂生长,没有分生胞子.对其18SrRNA基因进行了克隆测序并登录GenBank(AY758384).依据18SrRNA基因建立的系统进化树表明:该菌与Schizochytriumlimacinum具有紧密的亲源关系. 图7表2参29
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| 关 键 词： | 裂殖壶菌 二十二碳六烯酸 高度不饱和脂肪酸 18S rRNA 进化树分析 |
| 修稿时间： | 2004年10月27 |
ISOLATION OF SCHIZOCHYTRIUM SP. FJU-512 WITH HIGH YIELD OF DHA AND COMPARATIVE ANALYSIS ON ITS 18S rRNA GENE SEQUENCE |
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| Huang Jianzhong,JIANG Xianzhang.ISOLATION OF SCHIZOCHYTRIUM SP. FJU-512 WITH HIGH YIELD OF DHA AND COMPARATIVE ANALYSIS ON ITS 18S rRNA GENE SEQUENCE[J].Chinese Journal of Applied and Environmental Biology,2005,11(2):202-207. |
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| Authors: | Huang Jianzhong JIANG Xianzhang |
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| Abstract: | A strain FJU-512 highly producing docosahexaenoic acid (DHA) was isolated by using pine pollen as bait. Its single cell oil was very valuable since its polyunsaturated fatty acid contained only little docosapentaenoic acid but much DHA, which was up to 56.24% in the total fatty acids. The strain biomass of 33 g L -1 was obtained by high-density culture. Its growth was dependent on binary division without spores. Its 18S rRNA gene was successfully cloned by PCR and sequenced (GeneBank: AY758384). The phylogenetic tree based on 18S rRNA gene showed that Schizochytrium sp. FJU-512 was close to Schizochytrium limacinum . Fig 7, Tab 2, Ref 29 |
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| Keywords: | Schizochytrium docosahexaenoic acid polyunsaturated fatty acids 18S rRNA phylogenetic analysis |
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