| 酶联免疫吸附分析法测定水样中的毒死蜱 |
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| 引用本文： | 韩丽君,钱传范,李文明,江树人,王小如.酶联免疫吸附分析法测定水样中的毒死蜱[J].环境科学与技术,2006,29(3):35-37. |
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| 作者姓名： | 韩丽君 钱传范 李文明 江树人 王小如 |
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| 作者单位： | 1. 中国农业大学理学院,北京,100094
2. 河南农业大学植保学院,荆州,450002 |
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| 摘 要： | 自制了毒死蜱多克隆抗体,考察了离子强度和甲醇含量对抗原抗体竞争反应的影响,对反应体系进行了优化,最终建立了水样中毒死蜱的残留检测ELISA方法。结果表明,毒死蜱对抗体竞争性结合的I50为76.8μg/L,方法检出限为5.2μg/L。河水样品经简单前处理后分别采用ELISA和GC测定,用ELISA方法测得水样中毒死蜱的添加回收率为93.40%～102.1%,C.V为4.04%～5.18%,在水样中的最低检出浓度为0.08μg/L,检测灵敏度远高于GC,符合农药残留检测的要求。
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| 关 键 词： | 酶联免疫吸附测定法 毒死蜱 水样 残留检测 |
| 文章编号： | 1003-6504(2006)03-0035-03 |
| 收稿时间： | 2005-02-01 |
| 修稿时间： | 2005-04-14 |
Determination of Chlorpyrifos in Water by ELISA |
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| HAN Li-jun,QIAN Chuan-fan,LI Wen-ming,JIANG Shu-ren,WANG Xiao-ru.Determination of Chlorpyrifos in Water by ELISA[J].Environmental Science and Technology,2006,29(3):35-37. |
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| Authors: | HAN Li-jun QIAN Chuan-fan LI Wen-ming JIANG Shu-ren WANG Xiao-ru |
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| Abstract: | Using self-made polyclonal antibody the influence of ion-strength and the concentration of methanol on the competitive reaction were investigated,and the reaction system optimized.This paper developed an indirect competitive enzyme-linked immunosorbent assay(ELISA)for determining chloropyrifos residue in water.Surface water samples were detected with ELISA and GC after simple pre-processing with the detection limit of 0.08μg/L,result of ELISA being verified by GC method. |
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| Keywords: | chloropyrifos water smple pesticide residue |
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