| 除草剂莠去津对过氧化氢酶中酪氨酸微区的影响 |
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| 引用本文： | 刘维屏,杨炜春,刘惠君,陈学萍.除草剂莠去津对过氧化氢酶中酪氨酸微区的影响[J].环境科学,2002,23(6):86-90. |
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| 作者姓名： | 刘维屏 杨炜春 刘惠君 陈学萍 |
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| 作者单位： | 浙江大学环境科学研究所,杭州,310027 |
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| 基金项目： | 国家自然科学基金资助项目(39670420) |
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| 摘 要： | 应用紫外差光谱和荧光光谱研究了除草剂莠去津对过氧化氢酶中酪氨酸微区的影响.通过紫外差光谱发现,当9.1＜pH＜9.9之间变化时,差光谱吸收△A260nm急剧变化,酪氨酸酚羟基的表观离解常数pK侧链=9.5莠去津与过氧化氢酶作用后,可能是由于氢键的作用,紫外差光谱和荧光光谱都有一定的红移现象产生,但其表观离解常数未发生改变.SDS对其荧光性质有较大的影响;过氧化氢酶的荧光能被KI碰撞猝灭研究表明,酪氨酸残基可能是处于过氧化氢酶的亲水内核,莠去津可能与过氧化氢酶形成氢键,但对酪氨酸微区影响不大.
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| 关 键 词： | 莠去津 酪氨酸微区 过氧化氢酶 紫外差光谱 荧光光谱 |
| 文章编号： | 0250-3301(2002)06-05-0086 |
| 收稿时间： | 2001/10/3 0:00:00 |
| 修稿时间： | 2001年10月3日 |
Influence of Atrazine on the Tyrosine Environment of Catalase |
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| Liu Weiping,Yang Weichun,Liu Huijun and Chen Xueping.Influence of Atrazine on the Tyrosine Environment of Catalase[J].Chinese Journal of Environmental Science,2002,23(6):86-90. |
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| Authors: | Liu Weiping Yang Weichun Liu Huijun and Chen Xueping |
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| Institution: | Institute of Environmental Science, Zhejiang University, Hangzhou 310027, China. wpliu@mail.hz.zj.cn |
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| Abstract: | By the UV difference spectrum and fluorescence spectrum technique, the influence of atrazine on tyrosine environment of catalase was studied. According to the results of the UV difference spectrum curve, the UV difference adsorption delta A260nm showed changing rapidly when pH ranging from 9.1 to 9.9. UV difference spectrum and fluorescence spectrum of atrazine-catalase were red shift that maybe caused by effect of hydrogen bonding. The fluorescence spectrum was sensitive to SDS and quenchable by KI. The results indicated that the tyrosine residues of catalase reside in hydrophilic core, and there is hydrogen bonding between atrazine and catalase probably. Atrazine has little effect on tyrosine environment of catalase. |
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| Keywords: | atrazine tyrosine environment catalase UV difference spectrum fluorescence spectrum |
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