石油降解菌XD-1产表面活性剂的性能

从含油废水中筛选分离到1株原油降解菌XD-1,鉴定为假单胞菌(Pseuomonas sp.).初步实验表明菌XD-1具有较强的产表面活性剂乳化原油的作用,对该菌的产表面活性剂性能进行了研究.实验证明,菌XD-1所产表面活性剂为脂肽类物质,菌在生长对数期产表面活性剂,表面活性剂的产生为生长相关型;充足的碳源是产表面活性剂的必需条件,菌利用原油为碳源时能持续大量地产表面活性剂;原油和尿素为产表面活性剂的最适碳源和氮源,菌XD-1产表面活性剂的最佳营养培养基组成为葡萄糖10 g,尿素4 g,磷酸二氢钾1 g,微量元素液4 mL,水1 L,pH 8.0.

Characteristics of oil-degrading strain XD-1 releasing biosurfactants

An oil-degrading bacterium XD-1 was separated from oil wastewater and was identified as Pseuomonas sp.Preliminary experiment showed that XD-1 had high ability of releasing biosurfactant and emulsifying crude oil.The characteristics of the strain releasing biosurfactant were studied.The experimental results indicated that the biosurfactants were Lipo-peptide compounds,strain XD-1 released biosurfactants in its logarithmic growth period,thus the mode of growth-correlativity of the strain XD-1 releasing biosurfactant was proposed.Strain XD-1 could release a mass of biosurfactants continuously when growing in a medium containing crude oil as carbon source.The results showed that the best carbon source and nitrogen source were crude oil and urea,and the best fermentation conditions were as follows: glucose 10 g,urea 4 g,monopotassium phosphate 1 g,micro-elements 4 mL,water 1 L, pH 8.0.