土霉素降解菌TJ-1在猪粪无害化处理中的作用

利用普通理化分析结合Biolog微平板技术研究了土霉素高效降解菌Staphylococcus sp.TJ-1在新鲜猪粪无害化处理中的作用.结果表明,土霉素高效降解菌的接种能显著提高猪粪中土霉素的降解效率(p＜0.05),21d堆肥结束时可将土霉素降解率从62.7％提升至82.0％.堆肥结束后,不接种降解菌的普通堆肥工艺和接种降解菌的高效堆肥工艺中NH+4-N含量分别为189.34、42.36 mg·kg-1,NO-3-N含量分别为439.38、238.06 mg·kg-1.Biolog结果显示,土霉素降解菌TJ-1对土霉素中碳源的代谢有利于堆体中氨基酸类及芳香族化合物等氮源的降解,缓解了堆体中有毒有害物质对其他微生物的损伤,保证了微生物群落的多样性和活性,对维护堆体生态系统的稳定性具有良好作用.因此,接种高效降解菌进行堆肥是一种良好的消除抗生素残留的猪粪无害化工艺.

The role of oxytetracycline-degrading bacterium TJ-1 on the hazard-free treatment of pig manure

The role of oxytetracycline(OTC)-degrading bacterium Staphylococcus sp. TJ-1 on the harmless treatment of pig manure was evaluated by physio-chemical analysis and Biolog technique. The results showed that the inoculation of strain TJ-1 could significantly improve the degradation efficiency of OTC from 62.7% to 82.0% on 21 d. At the end of the compost, the concentration of NH₄⁺-N and NO₃^--N in the treatment with the strain TJ-1 inoculation were 42.36 and 238.06 mg·kg^-1, respectively; while they were 189.34 and 439.38 mg·kg^-1, respectively, in the treatment without the strain TJ-1 inoculation. Biolog analyses showed that the metabolism of OTC by strain TJ-1 could be beneficial to the degradation of nitrogen sources such as amino acids and aromatic compounds, which relieved the toxic effect of hazardous and noxious substances on other microorganisms and maintained the diversities and activities of bacterial community. This had a good effect on the balance of composting eco-system. Thus, the composting with the inoculation of high-efficient degrading bacteria is a good harmless treatment for pig manure to eliminate antibiotic residues.