稻草秸秆发酵液对铜绿微囊藻生化参数影响

针对铜绿微囊藻在稻草秸秆（水稻分蘖枝）发酵液胁迫下的酶学特征及抗氧化能力、藻毒素和多糖含量等生化指标进行了检测分析，旨在为利用稻草秸秆抑藻提供进一步理论依据.结果表明：铜绿微囊藻在水稻分蘖枝发酵液胁迫下，其表征藻细胞代谢水平的酯酶活性显著降低，实验第5d，最高浓度组（0.65%V/V）99.9%的藻细胞内酯酶活性均被抑制，超氧化物歧化酶（SOD）与谷胱甘肽过氧化物酶（GSH-Px）活力分别下降至同期对照组的11.03%和8.47%；丙二醛（MDA）含量则与发酵液浓度以及作用时间呈显著正相关，pearson分析表明，所有浓度组和作用时间内r值均大于0.9，而P值均小于0.01，表明水稻分蘖枝发酵液可显著降低藻细胞的抗氧化水平；但高浓度水稻分蘖枝发酵液没有引起微囊藻毒素（MCs）升高，甚至显著降低MCs和多糖的含量，与对照组相比，P<0.01.因此，水稻分蘖枝发酵液可通过影响铜绿微囊藻的代谢过程，降低藻细胞抗氧化以及抵御对环境胁迫的能力，从而达到有效抑藻的目的.

Effects of rice straw fermentation liquid on the biochemical parameters of Microcystis aeruginosa

The enzymatic characteristics, antioxidant capacity, microcystins and polysaccharide contents of Microcystis aeruginosa were investigated under the stress of rice straw (rice tillering branch) fermentation liquid, so as to provide a further theoretical basis for using rice straw to inhibit algae. The results showed that the esterase activity of M. aeruginosa under the stress of rice tiller branch fermentation liquid significantly reduced, which characterizes the level of algal cell metabolism. On the 5th day, 99.9% of the M. aeruginosa intracellular esterase activity was inhibited in the highest concentration group (0.65% V/V), the SOD and GSH-Px activities of the cells were only 11.03% and 8.47% of the control group in the same period. The MDA content was significantly positively correlated with the concentration of the fermentation liquid and the action time, pearson analysis showed that the r values were greater than 0.9 for all concentration groups, while the p values were less than 0.01, indicating that the rice tiller fermentation liquid can significantly reduce the antioxidant level of M. aeruginosa; but the high-concentration rice tiller fermentation liquid did not cause the increase of MCs, and even significantly reduce the content of MCs and polysaccharides, compared with the control group, P<0.01. Therefore, the fermentation liquid of rice tillering branches can affect the metabolic process of M.aeruginosa, and reduce the ability of the cells to resist oxidation and environmental stress, thereby it can achieve the purpose of effectively inhibiting M.aeruginosa