Heavy metals detection in river water with cantilever nanobiosensor |
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Authors: | Aline Andressa Rigo Alana Marie De Cezaro Daniela Kunkel Muenchen Janine Martinazzo Alexandra Manzoli Juliana Steffens |
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Institution: | Department of Food Engineering, URI - Erechim, Erechim, RS, Brazil |
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Abstract: | AbstractHeavy metals can be highly toxic depending on the dose and the chemical form. In this context, sensing devices such as nanobiosensors have been presented as a promising tool to monitor contaminants at micro and nanoscale. In this work, cantilever nanobiosensors with phosphatase alkaline were developed and applied to detect heavy metals (Pb, Ni, Cd, Zn, Co, and Al) in river water. The nanobiosensor surface was functionalized by the self-assembled monolayers (SAM) technique using 16-mercaptohexadecanoic acid, N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide (EDC) and N- hydroxysuccinimide (NHS), and phosphatase alkaline enzyme. The sensing layer deposited on the cantilever surface presented a uniform morphology, at nanoscale, with 80?nm of thickness. The nanobiosensor showed a detection limit in the ppb range and high sensitivity, with a stability of fifteen days. The developed cantilever nanobiosensor is a simple tool, suitable for the direct detection of contaminants in river water. |
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Keywords: | Self-assembled monolayers (SAM) surface detection limit phosphatase alkaline water |
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