Assessment of DNA damage in Brazilian workers occupationally exposed to pesticides: a study from Central Brazil |
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Authors: | Carolinne Borges Khayat Emília Oliveira Alves Costa Macks Wendhell Gonçalves Damiana Mirian da Cruz e Cunha Alex Silva da Cruz Caroline Oliveira de Araújo Melo Rogério Pereira Bastos Aparecido Divino da Cruz Daniela de Melo e Silva |
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Institution: | 1. Departamento de Biologia, Núcleo de Pesquisas Replicon, Pontifícia Universidade Católica de Goiás, Rua 235, n. 40, Departamento de Biologia, Bloco L, área IV, Setor Universitário, Goiania, Goiás, 74605-050, Brazil 2. Programa de Pós-Gradua??o em Ciências Gen?micas e Biotecnologia, Universidade Católica de Brasília, SGAN quadra 916, Módulo B, Av. w5 norte, Asa Norte, Brasília, Distrito Federal, 70790-160, Brazil 3. Instituto de Ciências Biológicas, Universidade Federal de Goiás, Campus Samambaia, Goiania, Goiás, 74001-970, Brazil
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Abstract: | We evaluated 41 rural workers occupationally exposed to pesticides and 32 subjects as a control group, using the micronucleus (MN) and the comet assay. For the comet assay, we evaluated the peripheral blood, and for the MN, we sampled cells from the oral epithelium. Damage to DNA was measured by tail length, % DNA in tail (% tail), olive tail moment (OTM), and tail moment (TM). The exposed group presented an 8× increase in MN frequency, when compared to the control group (p <0.05). When we contrasted the MN frequencies between the individuals that use and do not use personal protective equipment, we found a mean of 7.5 MN (57 % variance) and 12.1 MN (130 % variance), respectively. The binucleated cells were 0.04 and 0.005, in the exposed and control groups, respectively, indicating 8× increase in the number of binucleated cells, when comparing the groups (p <0.05). In the comet assay, we demonstrated statistically significant differences in three parameters (% DNA, OTM, and TM) indicating that the rural workers presented high levels of genomic damages. Our results indicate that occupational exposure to pesticides could cause genome damage in somatic cells, representing a potential health risk to Brazilian rural workers that deal constantly with agrochemicals without adequate personal protection equipment. |
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