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| 卤代烃毒性的化学评价法:鸟嘌呤卤代烃加合物FTIR、UV光谱表征 | |
| 引用本文： | 徐维并,佟柏龄,黄芹.卤代烃毒性的化学评价法:鸟嘌呤卤代烃加合物FTIR、UV光谱表征[J].环境科学,2001,22(1):67-70. |
| 作者姓名： | 徐维并 佟柏龄 黄芹 |
| 作者单位： | 1. 中国科学院生态环境研究中心,北京,100085 2. 中国地质科学院测试所,北京,100037 3. 中国科学院生态环境研究中心北京,100085 |
| 基金项目： | 国家自然科学基金资助项目(29777024) |
| 摘 要： | 研究鸟嘌呤与一系列卤代烃反应的加合产物 ,利用HPLC二极管阵列检测器得到的特征紫外光谱 (UV)对加合产物样品进行了鉴定 ,然后加合产物经薄层 (TLC)分离 ,再进一步做红外 (FTIR)鉴定 ,从而得到鸟嘌呤 卤代烃氧位和氮位加合物的红外光谱表征 .研究表明 ,鸟嘌呤卤代烃的氮位加合物异构体的紫外光谱吸收峰的波长在 240～250nm之间 ,氧位加合物UV波长在 260～270nm附近 .鸟嘌呤与本文涉及的各卤代烃的加合物的红外光谱之间差别不大 :其氮位加合物在 1700cm-1处 ,均有CO的特征吸收峰 ;其氧位加合物的红外光谱亦相近 . |
| 关 键 词： | 鸟嘌呤 加合物 异构体 FTIR |
| 文章编号： | 0250-3301(2001)01-04-0067 |
| 收稿时间： | 2000/2/21 0:00:00 |
| 修稿时间： | 2000年2月21日 |
Chemical assessment of mutagenicity of halohydrocarbons: FTIR, UV spectrum characteristics of guanine-halohydrocarbon adducts |
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| Xu Weibing,Tong Bailing and Huang Qin.Chemical assessment of mutagenicity of halohydrocarbons: FTIR, UV spectrum characteristics of guanine-halohydrocarbon adducts[J].Chinese Journal of Environmental Science,2001,22(1):67-70. | |
| Authors: | Xu Weibing Tong Bailing and Huang Qin |
| Institution: | Research Center for Eco-Environmental Science, Chinese Academy of Sciences, Beijing 100085, China. |
| Abstract: | Determination and identification of guanine-halohydrocarbon adduct isomers were carried out by high performance liquid chromatography (HPLC) and Fourier transform infrared spectroscopy (FTIR). The experimental results showed that the UV spectral overlay of O6-guanine adducts retrieved after HPLC at 260-270 nm. The UV spectra of N-side adducts are similarly between 240-250 nm. After thin layer chromatographic separation (silica gel-GF254), the all IR spectra of the N-side adducts of guanine with different halohydrocarbons showed a absorption near 1700 cm-1 assigned the characteristic peak of C=O. The IR spectra among the adducts of O6-guanine with different halohydrocarbons are quite similar. |
| Keywords: | guanine adduct isomer FTIR |
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