Effect of oxygen and salt on haemolymph oxygen binding in the brine shrimp Artemia franciscana |
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| Authors: | B De Wachter J Van Den Abbeele G Wolf |
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| Institution: | (1) Department of Biology, University of Antwerp (RUCA), Groenenborgerlaan 171, U7, 2020 Antwerp, Belgium;(2) Present address: Department of Biological Sciences, University of Calgary, 2500 University Drive NW, T2N 1N4 Calgary, Alberta, Canada;(3) Present address: Department of Entomology, Institute of Tropical Medicine, Nationalestraat, 2000 Antwerp, Belgium |
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| Abstract: | The oxygen-binding properties of haemolymph from laboratory-reared Artemia franciscana were investigated in vitro. Adult female brine shrimp without eggs were acclimated to seven different combinations of salinity and oxygen. The oxygen affinity (P50) of unprocessed haemolymph rises with acclimation oxygen partial pressure (PO2) up to normoxic values, after which no influence of oxygen occurs. The increase in P50 with acclimation PO2 can be explained by the change in proportion of each of three different haemoglobins in the haemolymph. Salinity acclimation has no effect on haemolymph P50. The effects of the different major salts NaNO3, NaCl, Ca(NO3)2, Mg(NO3)2], pH, and the metal-binding ligand EGTA on the oxygen-binding properties of buffered haemolymph (of shrimp acclimated to 10 salinity) were also studied. Little or no effect of these salts could be found. A small Bohr effect (pH 6.5 to 9.1,  =-0.11) was noted. Addition of EGTA caused a significant decrease of the oxygen affinity at concentrations up to 50 mmoll-1. |
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