| 一株高效降解废水有机质耐冷菌的筛选、鉴定及特性研究 |
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| 引用本文： | 高新新,何世颖,杨林章,徐流凤,曹慧. 一株高效降解废水有机质耐冷菌的筛选、鉴定及特性研究[J]. 环境科学学报, 2015, 35(4): 1006-1011 |
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| 作者姓名： | 高新新 何世颖 杨林章 徐流凤 曹慧 |
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| 作者单位： | 1. 南京农业大学农业部农业环境微生物工程重点开放实验室,南京,210095
2. 江苏省农业科学院农业资源与环境研究所,南京,210014 |
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| 基金项目： | 江苏省农业科技自主创新资金项目(No. CX(13)3039);国家自然科学基金项目(No.41301267) |
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| 摘 要： | 利用生物法进行污水处理已被国内外广泛使用,但由于温度影响微生物的生长和繁殖,使其成为生物污水处理效率的限制因子.本研究从污水处理厂活性污泥中分离出5株在低温4℃条件下生长良好的菌株,其中菌株DW1在4℃条件下,96 h时可将初始COD为800 mg·L-1的生活污水有机物去除率高达67.7%.结合菌落形态特征、生理生化特性以及16S r DNA序列分析,将菌株DW1初步鉴定为假单胞菌(Pseudomonas sp.).菌株DW1对COD负荷较高的污水处理效果较好;接种量为3%(体积分数)、p H为7.0时,菌株对污水COD去除率和DHA活性较高.在4℃和25℃条件下,菌株DW1均在96 h对污水COD去除率和DHA活性最高,分别为71.2%、11.35μg·m L·h-1(以TF计)和78.8%、15.11μg·m L-1·h-1(以TF计).
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| 关 键 词： | 耐冷菌 模拟污水 菌株鉴定 COD去除率 DHA活性 |
| 收稿时间： | 2014-06-25 |
| 修稿时间： | 2014-08-23 |
Isolation and identification of an efficient psychrotrophic bacterium and its characteristics of organic matter degradation |
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| GAO Xinxin,HE Shiying,YANG Linzhang,XU Liufeng and CAO Hui. Isolation and identification of an efficient psychrotrophic bacterium and its characteristics of organic matter degradation[J]. Acta Scientiae Circumstantiae, 2015, 35(4): 1006-1011 |
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| Authors: | GAO Xinxin HE Shiying YANG Linzhang XU Liufeng CAO Hui |
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| Affiliation: | Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Microbiology Department, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095;Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Sciences, Nanjing 210014;Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Sciences, Nanjing 210014;Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Microbiology Department, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095;Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Microbiology Department, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095 |
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| Abstract: | |
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| Keywords: | pychrotrophic bacteria simulation wastewater strain identification COD removal rate dehydrogenase activity |
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