| 白菜叶绿体DNA快速提取及分析 |
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| 引用本文： | 史公军,侯喜林,王彦华.白菜叶绿体DNA快速提取及分析[J].应用与环境生物学报,2005,11(3):283-285. |
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| 作者姓名： | 史公军 侯喜林 王彦华 |
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| 作者单位： | 1. 南京农业大学,作物遗传与种质创新国家重点实验室,南京,210095
2. 南京农业大学,园艺学院,南京,210095 |
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| 基金项目： | 国家自然基金项目资助(No.30370973)~~ |
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| 摘 要： | 采用SDS-蛋白酶法提取了白菜的叶绿体DNA.琼脂糖电泳及紫外分光光度分析表明,所提取的叶绿体DNA质量高,除cpDNA主环外,在胞质雄性不育新种质及原不育系中还存在约1375bp和831bp的两条质粒分子.用所提取的叶绿体DNA作为模板进行RAPD扩增,获得了清晰的扩增图谱.该方法与传统的提取方法相比省时、高效、无污染.图2表1参14
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| 关 键 词： | 白菜 SDS-蛋白酶法 叶绿体DNA 提取 |
| 修稿时间： | 2004年3月10日 |
EXTRACTION AND ANALYSIS OF CHLOROPLAST DNA IN NON-HEADING CHINESE CABBAGE |
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| SHI Gongjun,Hou Xilin,WANG Yanhua.EXTRACTION AND ANALYSIS OF CHLOROPLAST DNA IN NON-HEADING CHINESE CABBAGE[J].Chinese Journal of Applied and Environmental Biology,2005,11(3):283-285. |
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| Authors: | SHI Gongjun Hou Xilin WANG Yanhua |
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| Abstract: | Chloroplast DNA was extracted from young seedlings of non-heading Chinese cabbage by SDS-proteinase method. Its quality was evaluated by agrose gel electrophoresis and UV spectrophotometer. Except the main cpDNA band, two minor particle molecules about 1 375 bp and 831 bp appeared in the profile of the original and new CMS line. RAPD analysis was made with the cpDNA as template and clear amplification profile was obtained. This method was proved to be more efficient, more time-saving and less pollution than traditional methods. Fig 2, Tab 1, Ref 14 |
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| Keywords: | non-heading Chinese cabbage SDS-proteinase method cpDNA extraction |
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