石棉表面改性对石棉诱导人胚肺细胞产生羟基自由基的影响

为了研究用柠檬酸铝、混合稀土或亚硒酸钠预处理温石棉对石棉诱导人胚肺(HEL)细胞产生羟基自由基(·OH)的影响,笔者用不同浓度的柠檬酸铝、混合稀土或亚硒酸钠溶液浸泡温石棉1小时后,再将其与人胚肺细胞共同孵育,利用电子自旋捕获法测定反应体系中羟基自由基信号。结果显示,石棉可诱导人胚肺细胞产生羟基自由基,并且所产生的自由基信号(峰高)随石棉浓度升高而增强,呈一定的剂量依赖性。经3种化合物预处理的温石棉组人胚肺细胞所产生的自由基信号(峰高)均明显低于未处理温石棉组。上述结果表明,温石棉可诱导人胚肺细胞产生羟基自由基,但用3种化合物预处理温石棉,均可抑制温石棉诱导人胚肺细胞产生羟基自由基。

Inhibitory Effect of Surface Modification on Chrysotile-induced Prodution of OH in Human Embryo Lung Cells

To study the effects of native chrysotile and compounds-pretreated chrysotile on the production of hydroxyl radical ( ·OH)in human embryo lung (HEL) cells, the chrysotile, after being soaked in aluminium citrate, mixed rare earths or sodium selenite solutions at different concentrations for 1 hour, were incubated with HEL cells for 10min., then the levels of signal of ·OH were determined by using electron spin resonance(ESR). The results showed that the HEL cells were exposed to native chrysotile at 0.125, 0.25, 0.5, 1.0μg/ml respectively for 10min., the levels of signal of ·OH increased compared with control, and showed a good dose-effect relationship. But less increase could be found when cells were exposed to compounds-pretreated chrysotile. These results indicted that chrysotile can induce production of ·OH in HEL cells, while the pretreatment of chrysotile with aluminium citrate, mixed rare earths or sodium selenite solutions can inhibit chrysotile-induced production of ·OH in HEL cells.