Investigation of the effects of static magnetic field on apoptosis in bone marrow stem cells of rat |
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| Authors: | Zeinab Tavasoli Parviz Abdolmaleki Seyed Javad Mowla Faezeh Ghanati and Amir Sabet Sarvestani |
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| Institution: | (1) Department of Biophysics, Faculty of Science, Tarbiat Modares University, Tehran, Iran;(2) Department of Genetics, Faculty of Science, Tarbiat Modares University, Tehran, Iran;(3) Department of Plant Science, Faculty of Science, Tarbiat Modares University, Tehran, Iran |
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| Abstract: | This research was carried out to evaluate the influence of static magnetic field on the rate of apoptosis in bone marrow stem
cells (BMSCs) of rats. Extracted cells were suspended in αMEM as a culture media for 48 h. After that, cells were exposed
to 15 mT static magnetic field (SMF) for 5 h, incessantly with or without FeCl2. The rate of apoptosis was then assessed via flow cytometery. The results showed that either treatment with FeCl2 or exposure to SMF enhanced the rate of apoptotic cells. Moreover, cells that were treated simultaneously with FeCl2 and SMF have higher rate of apoptosis. An increase in apoptosis by 26.5% was induced by SMF alone and an increase in apoptosis
by 28.2% was induced by a combination of FeCl2 and SMF, compared to their corresponding controls. The results recommended that the effects of SMF on apoptosis may be related
to increment of the number of free radicals in the cells. |
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| Keywords: | Stem cell Apoptosis Static magnetic field FeCl2 Flow cytometry |
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