| 小麦抗禾谷类根线虫基因Rkn-mnl 的SCAR标记 |
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| 引用本文： | 闫乃红,陈静,马欣荣,邓光兵,余懋群.小麦抗禾谷类根线虫基因Rkn-mnl 的SCAR标记[J].应用与环境生物学报,2003,9(3):250-253. |
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| 作者姓名： | 闫乃红 陈静 马欣荣 邓光兵 余懋群 |
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| 作者单位： | 中国科学院成都生物研究所,成都,610041 |
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| 基金项目： | 中国科学院知识创新工程重要方向性项目~~ |
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| 摘 要： | 将2个与小麦抗禾谷类根线虫基因Rkn-mnl紧密连锁的RAPD标记分别克隆、测序，根据测序结果设计两对特异PCR引物，通过SCAR-PCR反应条件优化，成功地将特异RAPD标记OPYl6-1065转换成了稳定的SCAR标记．图4参14
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| 关 键 词： | 小麦 抗禾谷类根线虫基因 Rkn－mnl SCAR标记 克隆 测序 |
| 修稿时间： | 2002年11月18 |
CONVERSION OF RAPD MARKER TO SCAR MARKER OF WHEAT CEREAL ROOT-KNOT NEMATODE-RESISTANT GENE Rkn-mnl |
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| YAN Naihong,CHEN Jing,MA Xinrong,DENG Guangbing & YU Maoqun.CONVERSION OF RAPD MARKER TO SCAR MARKER OF WHEAT CEREAL ROOT-KNOT NEMATODE-RESISTANT GENE Rkn-mnl[J].Chinese Journal of Applied and Environmental Biology,2003,9(3):250-253. |
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| Authors: | YAN Naihong CHEN Jing MA Xinrong DENG Guangbing & YU Maoqun |
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| Institution: | YAN Naihong,CHEN Jing,MA Xinrong,DENG Guangbing & YU Maoqun ** |
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| Abstract: | Two special RAPD markers linked with gene resistant to wheat cereal root knot nematode were cloned and sequenced respectively.The SCAR primers were designed according to the obtained sequences,and the SCAR PCR reaction conditions were optimized.Consequently,RAPD marker OPY16 1065 was successfully converted into SCAR marker. Fig 4,Ref 14 |
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| Keywords: | Rkn mnl molecular markers RAPD SCAR |
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