Dynamic changes of microbial community diversity in a photohydrogen
producing reactor monitored by PCR-DGGE |
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Authors: | YING Yanling LV Zhenmei MIN Hang and CHENG Jun |
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Institution: | 1. Institution of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou 310058, China 2. State Key Lab of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China |
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Abstract: | A PCR-DGGE (denaturing gradient gel electrophoresis of polymerase chain reaction) protocol was used for monitoring the dynamic changes in the microbial population during photohydrogen production. Total DNA was extracted directly from the mixed bacterial community in the reactor and subjected to PCR with V3-16S rDNA and pufM gene primers, and the amplifications were then analyzed by DGGE. The DGGE patterns demonstrated the dynamics of community structure and the shift of microbial diversity, which correspond... |
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Keywords: | PCR-DGGE V3-16S rDNA region pufM gene photohydrogen producing reactor |
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