环境浓度下邻苯二甲酸二乙酯对秀丽隐杆线虫的生态毒理效应

DEP (diethyl phthalate，邻苯二甲酸二乙酯)是一种在环境中普遍存在的具有潜在神经和生殖毒性的增塑剂，其会干扰环境生物的发育水平、运动行为及生化水平.以秀丽隐杆线虫的体长、体宽、头部摆动频率及生化指标作为测试终点，评估环境中DEP对秀丽隐杆线虫的生态毒理效应.在不同环境浓度(0、0.000 2、0.002、0.02、0.2、2 mg/L)的DEP溶液中，对秀丽隐杆线虫进行不同时间(24 h、72 h和10 d)的暴露试验.结果表明:①在不同浓度DEP溶液中暴露24 h后，秀丽隐杆线虫的发育水平均未受到显著影响(P>0.05).②在不同浓度DEP溶液中暴露72 h后，秀丽隐杆线虫的发育水平受到干扰.与对照组相比，在DEP浓度最高(2 mg/L)时，秀丽隐杆线虫体长下降了3.21%(P < 0.05)；在DEP浓度为0.000 2 mg/L时，秀丽隐杆线虫的体宽呈上升趋势，且头部摆动频率明显加快了5.52%(P < 0.01)，秀丽隐杆线虫的发育水平受到显著抑制.③在不同浓度DEP溶液中暴露10 d后，与对照组相比，秀丽隐杆线虫的体长、体宽和头摆频率均出现下降；在DEP浓度为2 mg/L时，秀丽隐杆线虫体内活性氧自由基、脂褐素和细胞凋亡水平累积均增长显著，出现氧化应激损伤.研究显示，不同环境浓度的DEP溶液会对秀丽隐杆线虫产生一定的毒性影响，且不同暴露时间和暴露浓度产生的毒性影响也存在一定差异. 

Ecotoxicological Effects of Diethyl Phthalate on Caenorhabditis elegans under Environmentally Relevant Concentrations

Diethyl phthalate (DEP) is a typical plasticizer that has been widely detected in the environment. DEP possesses potential neurological and reproductive toxicities, which can affect the development motion behavior as well as certain biochemical parameters of organisms. The present study was designed to evaluate the eco-toxicity of DEP under different exposure scenarios (24 h, 72 h and 10 d) at environmentally relevant concentrations (0, 0.0002, 0.002, 0.02, 0.2, 2 mg/L) on Caenorhabditis elegans, a typical model animal. To this end, the body length/width, head thrashing frequency, and biochemical indicators of C. elegans were applied as test endpoints to evaluate the ecotoxicological effects of DEP. The results revealed that under 24 h exposure scenario, compared to the control group, DEP exhibited no distinct effect on the development of C. elegans at all exposure levels (P>0.05). In the case of 72 h exposure, DEP was found to inhibit the development of C. elegans compared with the control group. Specifically, the body length declined by 3.21% (P<0.05) at a DEP level of 2 mg/L. Under 0.0002 mg/L DEP exposure, the body width was found to increase. Head thrashing frequency displayed stimulating effect when exposed to low DEP levels (<0.2 mg/L). When it was 0.0002 mg/L, it increased by 5.52% (P<0.01). In addition, the physiological endpoints, including body length, body width and head thrashing frequency, decreased significantly by comparison with the control group at all exposure levels after 10 d exposure. Moreover, the levels of reactive oxygen species, lipofuscin and apoptosis increased significantly at 2 mg/L. The results revealed that DEP exposure exhibited different toxicological effects on C. elegans, depending on the concentration and exposure scenarios.