贫营养条件下2种MBR污泥微生物的菌群对比

为了揭示贫营养环境下MBR污泥微生物群落结构的演替和菌群变化的异同,取洗浴再生水、工业再生水MBR的污泥进行周期培养,利用PCR-DGGE和克隆测序技术获得了DNA指纹图谱并建立系统发育树。研究表明,微生物群落结构在贫营养条件下演替明显,洗浴水污泥微生物形成新的优势菌群(Uncultured Pseudomonas)而工业水只维持了原有的部分菌群(Uncultured Sphaerotilus)。2种污泥培养过程中种群多样性变化突出且差异显著。同时洗浴水污泥菌群相似性在培养第8天时发生突变而工业水总体变化平缓。克隆测序表明2种MBR污泥中既有与贫营养环境适生的共性种属又有与各自来源相对应的特性种属。菌群特异性与废水来源紧密相关,是造成2种污泥对贫营养环境适应能力不同的根本原因。

Comparative microbial community of MBR sludge in oligotrophic environment

In order to reveal the the succession of microbial community structure and difference on flora change, period cultivation under oligotrophic environment was undertook using sludge of MBRs treating bathing reclaimed water and industrial reclaimed water. The technique of PCR-DGGE, clone and sequence were applied to obtain DNA fingerprint profile and construct phylogenetic tree. Results indicated that both community structure had obvious succession in oligotrophic environment.The microorganism of MBR treating bathing reclaimed water formed new dominant species, but that treating industrial reclaimed water reserved some of original species. The population diversity of the two types of sludge changed significantly and had notable difference. Meanwhile the similarity change on bathing sludge had mutation on the eighth day, the other one changed smoothly overall. There was not only common species adapting to oligotrophic environment but also specific species between the two types of sludge. The flora specificity is closely related to the wastewater sources causing the difference of adaptaion ability.