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染料浓度和盐度对白腐真菌同工酶的电泳分析
引用本文:武琳慧,黄民生,施华宏.染料浓度和盐度对白腐真菌同工酶的电泳分析[J].环境科学与技术,2007,30(11):7-9.
作者姓名:武琳慧  黄民生  施华宏
作者单位:华东师范大学资源与环境科学学院,上海,200062
摘    要:应用聚丙烯酰胺凝胶电泳技术分析,并比较了染料废水的两大因子(染料浓度和盐度)对黄孢原毛平革菌过氧化物同工酶谱带的影响程度,结果表明:在染料或盐胁迫下,黄孢原毛平革菌均表现出应急产酶的响应,虽然过氧化物同工酶谱带数量未发生增加或减少,但谱带强度受到较大影响,活性艳红X-3B浓度≤200mg/L或NaCl浓度≤15g/L时均能诱导过量产酶,继续提高染料浓度或盐度将导致产酶抑制,染料浓度对酶带的影响作用高于盐度。

关 键 词:白腐真菌  染料  氯化钠  浓度  同工酶谱带
文章编号:1003-6504(2007)11-0007-03
修稿时间:2006-10-23

Effect of Dye and Salt Concentration on Peroxidase Isoenzyme Pattern of White Rot Fungi
WU Lin-hui,HUANG Min-sheng,SHI Hua-hong.Effect of Dye and Salt Concentration on Peroxidase Isoenzyme Pattern of White Rot Fungi[J].Environmental Science and Technology,2007,30(11):7-9.
Authors:WU Lin-hui  HUANG Min-sheng  SHI Hua-hong
Abstract:Isoenzyme patterns of peroxidase of Phanerochaete chrysosporium under different dye or salt concentration were studied. Lower concentration of reactive brilliant red X-3B or NaCl could obviously induced over-production of peroxidase, whereas high concentration of reactive brilliant red X-3B less than 200mg/L had greatly inhibitive effect on peroxidase production. Results indicated that Phanerochaete chrysosorium had undergone a strong response for peroxidase production to dye and salt concentrations, that was inducement, inhibition and exhaustion.
Keywords:white rot fungi  dye  NaCl  concentration  isoenzyme pattern
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