| 一株Zn抗性菌株的筛选鉴定及吸附条件优化 |
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| 引用本文： | 李慧芬,林雁冰,王娜娜,郭军康,韦革宏.一株Zn抗性菌株的筛选鉴定及吸附条件优化[J].环境科学学报,2010,30(11):2189-2196. |
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| 作者姓名： | 李慧芬 林雁冰 王娜娜 郭军康 韦革宏 |
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| 作者单位： | 西北农林科技大学生命科学学院,陕西省农业分子生物学重点实验室,杨凌,712100 |
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| 基金项目： | 国家自然科学基金(No.30670372, 30970003, 30900215) |
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| 摘 要： | 采用浓度梯度法从铅锌尾矿区与污水灌溉区土壤中筛选了一株Zn抗性放线菌,并通过形态与培养特征、生理生化特性、细胞壁组分与16SrRNA基因序列分析对该菌株进行了鉴定.同时,综合运用单因素试验与正交试验法对该菌株吸附Zn2+的条件进行了优化.结果表明,菌株CCNWHX72-14经鉴定为高加索链霉菌(Streptomyces ciscaucasicus),其对Zn2+的抗性达到845mg·L-1.锌胁迫生长曲线表明,该菌株在130mg·L-1Zn2+胁迫时生长良好,且最适培养时间为7d.各个单因素条件对该菌株吸附Zn2+的影响顺序为:接种量初始Zn2+浓度初始pH转速温度.该菌株吸附Zn2+的最佳条件为:初始Zn2+浓度为150mg·L-1,初始pH=5,接种量为1%,转速为60r·min-1,温度为28℃.菌株CCNWHX72-14在最佳条件下对Zn2+的吸附较具有良好的重复性与稳定性,最高吸附量可达51.05mg·g-1,该研究为进一步探讨链霉菌的Zn2+吸附机制及其在生物修复中的应用奠定了基础.
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| 关 键 词： | 高加索链霉菌 锌 吸附 单因素 正交 |
| 收稿时间： | 2/9/2010 10:30:22 AM |
| 修稿时间： | 4/27/2010 8:21:07 AM |
Screening and identification of a heavy metal-resistant strain and optimization of its zinc biosorption conditions |
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| LI Huifen,LIN Yanbing,WANG Nan,GUO Junkang and WEI Gehong.Screening and identification of a heavy metal-resistant strain and optimization of its zinc biosorption conditions[J].Acta Scientiae Circumstantiae,2010,30(11):2189-2196. |
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| Authors: | LI Huifen LIN Yanbing WANG Nan GUO Junkang and WEI Gehong |
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| Institution: | College of Life Sciences, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University, Yangling 712100,College of Life Sciences, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University, Yangling 712100,College of Life Sciences, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University, Yangling 712100,College of Life Sciences, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University, Yangling 712100 and College of Life Sciences, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University, Yangling 712100 |
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| Abstract: | |
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| Keywords: | Streptomyces ciscaucasicu zinc biosorption single factors orthogonal |
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