Delignification of disposable wooden chopsticks waste for fermentative hydrogen production by an enriched culture from a hot spring |
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| Authors: | Kanthima Phummal Tsuyoshi Imai Alissara Reungsang Prapaipid Chairattanamanokorn Masahiko Sekine Takaya Higuchi Koichi Yamamoto and Ariyo Kanno |
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| Institution: | Division of Environmental Science and Engineering, Graduate School of Science and Engineering, Yamaguchi University, Yamaguchi 7558611, Japan;Division of Environmental Science and Engineering, Graduate School of Science and Engineering, Yamaguchi University, Yamaguchi 7558611, Japan;Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40000, Thailand;Environmental Technology Unit, Department of Environmental Science, Kasetsart University, Bangkok 10903, Thailand;Division of Civil and Environmental Engineering, Graduate School of Science and Engineering, Yamaguchi University, Yamaguchi 7558611, Japan;Division of Environmental Science and Engineering, Graduate School of Science and Engineering, Yamaguchi University, Yamaguchi 7558611, Japan;Division of Civil and Environmental Engineering, Graduate School of Science and Engineering, Yamaguchi University, Yamaguchi 7558611, Japan;Division of Civil and Environmental Engineering, Graduate School of Science and Engineering, Yamaguchi University, Yamaguchi 7558611, Japan |
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| Abstract: | Hydrogen (H2) production from lignocellulosic materials may be enhanced by removing lignin and increasing the porosity of the material prior to enzymatic hydrolysis. Alkaline pretreatment conditions, used to delignify disposable wooden chopsticks (DWC) waste, were investigated. The effects of NaOH concentration, temperature and retention time were examined and it was found that retention time had no effect on lignin removal or carbohydrate released in enzymatic hydrolysate. The highest percentage of lignin removal (41%) was obtained with 2% NaOH at 100°C, correlated with the highest carbohydrate released (67 mg/gpretreated DWC) in the hydrolysate. An enriched culture from a hot spring was used as inoculum for fermentative H2 production, and its optimum initial pH and temperature were determined to be 7.0 and 50°C, respectively. Furthermore, enzymatic hydrolysate from pretreated DWC was successfully demonstrated as a substrate for fermentative H2 production by the enriched culture. The maximum H2 yield and production rate were achieved at 195 mL H2/g total sugarsconsumed and 116 mL H2/(L·day), respectively. |
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| Keywords: | alkaline pretreatment disposable wooden chopsticks waste fermentative hydrogen production hot spring enriched culture |
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