| pH和铜离子对微囊藻毒素-LR荧光免疫检测的影响与消除 |
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| 引用本文： | 龙峰,施汉昌,朱安娜,何苗. pH和铜离子对微囊藻毒素-LR荧光免疫检测的影响与消除[J]. 环境科学, 2009, 30(7): 1974-1977 |
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| 作者姓名： | 龙峰 施汉昌 朱安娜 何苗 |
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| 作者单位： | 清华大学环境科学与工程系,环境模拟与污染控制国家重点联合实验室,北京,100084;清华大学环境科学与工程系,环境模拟与污染控制国家重点联合实验室,北京,100084;清华大学环境科学与工程系,环境模拟与污染控制国家重点联合实验室,北京,100084;清华大学环境科学与工程系,环境模拟与污染控制国家重点联合实验室,北京,100084 |
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| 基金项目： | 国家高技术研究发展计划（863）青年基金项目（2005AA641040） |
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| 摘 要： | 研究了pH和铜离子对倏逝波全光纤免疫传感器检测微囊藻毒素-LR（MC-LR）的影响及其消除措施.研究表明,过酸或过碱条件对MC-LR免疫检测均有较强烈的影响,当pH<6或pH>8时,系统检测的信号随pH的降低或增大明显下降;而当pH在6～8之间时,检测标准曲线的IC50为1.01～1.04 μg/L,检测区间在0.12～10.5 μg/L之间,较适合MC-LR的检测.低浓度铜离子对MC-LR免疫检测的影响不大,当CuSO4浓度>5 mg/Ｌ时,系统检测荧光信号明显下降,而当CuSO4浓度达到10 mg/Ｌ时,系统检测信号下降70％以上.在预反应混合物中添加1%的螯合剂EDTA,能有效抑制铜离子对免疫检测的影响.
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| 关 键 词： | 微囊藻毒素.LR 铜离子 荧光免疫检测 倏逝波 光纤免疫传感器 |
| 收稿时间： | 2008-08-04 |
| 修稿时间： | 2008-10-20 |
Effects of pH and Cu2+ on the Detection of the Microcystin-LR Based on Fluorescence Immunoassay and Their Elimination |
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| LONG Feng,SHI Han-chang,ZHU An-na and HE Miao. Effects of pH and Cu2+ on the Detection of the Microcystin-LR Based on Fluorescence Immunoassay and Their Elimination[J]. Chinese Journal of Environmental Science, 2009, 30(7): 1974-1977 |
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| Authors: | LONG Feng SHI Han-chang ZHU An-na HE Miao |
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| Affiliation: | State Key Joint Laboratory of Environmental Simulation and Pollutants Control;Department of Environmental Science and Engineering;Tsinghua University;Beijing 100084;China |
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| Abstract: | The effects of pH and Cu 2+ on the detection of the Microcystin-LR based on evanescent wave all-fiber immunosensor (EWAI) and their elimination were studied. The experimental results indicated that both low pH and high pH greatly affected the immunoassay results of MC-LR. When pH was less than 6 or higher than 8,the fluorescence signals detected by immunosensor system obviously reduced with the decrease or increase of pH. While pH ranged from 6 to 8,IC 50 and the linear working range of MC-LR calculated fro... |
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| Keywords: | microcystin-LR Cu2+ fluorescence immunoassay evanescent wave fiber-optic immunosensor |
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