| RAPD方法鉴定油菜“杂油59”种子纯度的研究 |
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| 引用本文： | 刘杰,刘公社,齐冬梅,李殿荣,王灏,王道杰.RAPD方法鉴定油菜“杂油59”种子纯度的研究[J].应用与环境生物学报,2000,6(6):526-529. |
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| 作者姓名： | 刘杰 刘公社 齐冬梅 李殿荣 王灏 王道杰 |
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| 作者单位： | 1. 中国科学院植物研究所北京 100093
2. 陕西农垦科研中心陕西大荔 714000 |
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| 摘 要： | 本文采用ＲＡＰＤ分子标记技术对我国育成的油菜新品种“杂油５９”的亲本和Ｆ１代种子纯度进行了技术鉴定。用４０个１０ｍｅｒ的随机引物对“杂油５９”的２个亲本“垦Ｃ８”和“陕３Ａ”进行了ＲＡＰＤ分析，共出现２９０条带，分布于３５３０～２２０ｂｐ之间，大部分条带在双亲之间是相同的。引物Ｏｐａ－Ｏ６，Ｏｐｃ－１２，Ｏｐｃ－２０，Ｏｐｋ－０３，ｏｐｋ－１３，Ｏｐｊ－１２出现差异扩增带，经实验确认Ｏｐｋ－０３的
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| 关 键 词： | 油菜 种子纯度 RAPD 杂油59 |
| 修稿时间： | 1998年5月11日 |
IDENTIFICATION OF GENETIC PURITY OF SEED OF HYBRID RAPE “ZAYOU No.59” WITH RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) |
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| LIU Jie,LIU Gongshe,Qi Dongmei,Li Dianrong,WANG Hao,WANG Daojie.IDENTIFICATION OF GENETIC PURITY OF SEED OF HYBRID RAPE “ZAYOU No.59” WITH RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD)[J].Chinese Journal of Applied and Environmental Biology,2000,6(6):526-529. |
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| Authors: | LIU Jie LIU Gongshe Qi Dongmei Li Dianrong WANG Hao WANG Daojie |
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| Abstract: | The identification of the genetic purity of F1 hybrid seeds in new variety, rape “Zayou No59” was made via poly-morphic RAPD (Random Amplified polymorphic DNA) markers. Using 40 ten mer arbitrary oligonucheotide primers the parents of “Zayou No. 59” were analyzed will RAPD. Approximately 290 products, ranging from 220～3530 base pairs(bp), were amplified. The majority of the products were identical in both “Ken-C8” and “Shan-3A”. Primer Opa-06, Opc-12, Opc-20, Opk-03, Opk-13, Opj-12 generated polymorphic products. It was found that the reproducibility of primer Opk-03 was steady after repeating the tests. It could produce 5 bright bands, the fragment at 510 bp unique to the female line, and the fragment at 260 bp unique to the male line. The two fragments were complementary and stable in the F1 generation.The sampling selection of DNA sample used in identification of the purity in F1 was discussed in this report. Fig 3, Ref 11 |
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| Keywords: | rape purity of seed RAPD |
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