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Studies on the Measurement of Fluoride in Air and Plant Tissues By the Willard-Winter and Semiautomated Methods
Authors:Jay S Jacobson  D C Mccune  Leonard H Weinstein  Richard H Mandl  A E Hitchcock
Institution:1. Associate Plant Biochemist , Boyce Thompson Institute for Plant Research, Inc. , Yonkers , New York , USA;2. Associate Plant Physiologist , Boyce Thompson Institute for Plant Research, Inc. , Yonkers , New York , USA;3. Program Director for Plant Chemistry , Boyce Thompson Institute for Plant Research, Inc. , Yonkers , New York , USA;4. Assistant Chemist , Boyce Thompson Institute for Plant Research, Inc. , Yonkers , New York , USA;5. Plant Physiologist , Boyce Thompson Institute for Plant Research, Inc. , Yonkers , New York , USA
Abstract:Determinations of F in plant tissues by the Willard-Winter and semiautomated methods have been studied for the presence of determinate and indeterminate errors by multiple linear regression analysis. The results have provided a better understanding of the magnitude of differences between tissue samples required for statistical significance and have suggested that the errors involved are much greater both in number and magnitude than usually assumed. The results have also established that the semiautomated method is a satisfactory alternative to the Willard-Winter method for determining the F content of plant tissues. Investigations of the sources of error in F determinations by the semi-automated method were carried out, and the results indicated a number of ways of reducing errors. Determinations of the F content of air by three methods were compared and studied to estimate the magnitude and locate the sources of error. Here, too, the results indicated that present estimates of the reliability of determinations of the F content of air may be overrated, and they have suggested that improvement in the means of collection of HF is the best way of improving reproducibility.
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