分子生物学在生物强化处理环境污染物中的应用

综述了分子生物学的相关技术在生物强化处理环境污染物中的应用，主要包括：通过分子生物技术育种、筛选、构建高效降解污染物微生物菌株；提供更科学、快捷、准确多样的环境监测和环境评价技术手段；探索微生物与环境污染物间的相互关系，及微生物降解污染物的有关基因定位，相关主要技术有：核酸探针检测、DNA-DNA杂交，rRNA和rRNA基因序列分析。质粒指纹图谱分析、聚合酶链式反应（PCR）、低分子量PNA分布图及由PCR技术发展而带动的基因多态性技术，如变性梯度凝胶电泳（DGGE）、随机扩增多态性DNA技术（RAPD）等。

Application of Molecular Biology Technique to Treating Pollutants by Bioaugmentation

This article summarized the application of correlative molecular biology techniques to bioaugmentation for treating wastewater, including, screening and constructing the highly effective degradation microorganism strains, providing faster and more accurate monitoring and evolving methods based on PCR, studying on relationship between microorganisms and contaminated environment, and positioning of pollutant degrading genes of microorganisms. The related techniques including nucleic acid probe hybridization detection technique, DNA-DNA hybridization, rRNA and rRNA gene sequence analysis, plasmid finger-print analysis, polymerase chain reaction (PCR), Denaturing gradient gel electrophoresis (DGGE), randomly amplified polymorphic DNA (RAPD), low molecular weight RNA profile and so on.