制药废水厂抗性基因和微生物群落相关性研究

以2座制药废水厂的生物曝气阶段为例，结合Miseq测序分析技术和荧光定量PCR技术研究活性污泥中微生物群落和β-内酰胺类抗性基因的分布特征、扩增情况及其相关性.结果表明：β-内酰胺类抗性基因OXA-1、OXA-2和OXA-10在2个水厂（J厂和K厂）中均能被检出，OXA型基因丰度在K厂中为5.82×10⁵~3.94×10⁷copies/g（干重），在J厂的丰度范围为4.84×10⁷~1.09×10¹⁰copies/g，3种基因丰度在曝气处理中显著扩增.Miseq测序结果表明：K厂中主要优势菌门为Proteobacteria，Planctomycetes，Bacteroidetes，Chloroflexi和Acidobacteria等，总平均相对丰度比例为82.13%；J厂中主要优势菌门Proteobacteria，Bacteroidetes，Verrucomicrobia，Gemmatimonadetes和Thermi，总平均相对丰度比例为85.76%.冗余分析显示：生物群落中Bdellovibrio、KD8-87和Paracoccus等菌属可能是OXA-1、OXA-2和OXA-10的主要携带菌属；Hyphomicrobium、Thermomonas和Comamonadaceae可能是OXA-1的主要携带菌属，Caldilineaceae、Myxococcales和Pirellulaceae可能是OXA-10的主要携带菌属.

Correlation study between resistance genes and microbial communities in pharmaceutical wastewater treatment plants

The distribution characteristics, amplification and correlation of microbial communities and β-lactam resistance genes in active sludge were studied by Miseq sequencing analysis and fluorescence quantitative PCR. The samples of active sludge were obtained from the biological aeration stages of two pharmaceutical wastewater treatment plant (Plant J and K). β-lactam resistance genes OXA-1, OXA-2 and OXA-10can be detected in both plants. The abundance of OXA gene was 5.82×10⁵~3.94×10⁷copies/g (dry weight) in K plant and was 4.84×10⁷~1.09×10¹⁰copies/g in J plant. The Abundance of three genes in aeration treatment is significantly amplified. Miseq sequencing showed that the main dominant bacterium were Proteobacteria, Planctomycetes, Bacteroidetes, Chloroflexi and Acidobacteria in K plant and the total average relative abundance ratio was 85.76%. The main dominant bacterium were Proteobacteria, Bacteroidetes, Verrucomicrobia, Gemmatimonadetes and Thermi in J plant, the total average relative abundance ratio was 85.76%. Redundancy analysis display showed that Bdellovibrio、KD8-87 and Paracoccus in biological community were the major distribution factors of OXA-1、OXA-2 and OXA-10. Hyphomicrobium、Thermomonas and Comamonadaceae were the major distribution factor of OXA-1. Caldilineaceae、Myxococcales and Pirellulaceae were the major distribution factor of OXA-10.