裂褶菌F17对偶氮染料刚果红的脱色降解及其产物分析

利用本实验室新构建的染料脱色降解体系,对裂褶菌F17(Schizophyllum sp.F17)脱色降解刚果红进行了研究,分析了该菌的主要降解酶,并对刚果红降解产物进行分离和鉴定.结果表明,裂褶菌F17在此体系中对刚果红表现出较高的脱色降解能力,菌球加入48h后脱色率达到91.5%;酶活检测表明,该菌主要产生锰过氧化物酶(MnP),并在脱色48h时,MnP酶活达到最大值96.1U·L-1.此外,对脱色96h和192h后的脱色液进行紫外-可见扫描,发现刚果红在可见光区495nm处的吸收峰已消失,并在紫外区出现多个吸收峰.通过高效液相色谱分离得到1种刚果红降解产物,用质谱和傅立叶红外光谱鉴定,发现该产物相对分子量为184.2,主要官能团为-C6H4-和芳基-NH2,结合刚果红结构和该产物的核磁共振波谱推断其为联苯胺;并且随着降解时间的延长,联苯胺逐渐被降解.

Decolorization and degradation of Congo Red by Schizophyllum sp.F17 and catabolite analysis

Congo Red (CR) was decolorized and degraded by the main enzymes secreted by Schizophyllum sp. F17 which were investigated in a novel system established by our laboratory. Catabolite analysis showed that CR was decolorized and degraded effectively by Schizophyllum sp. F17. The decolorization percentage of CR reached 91.5% 48 h after mycelial pellet addition and the maximum activity of MnP, the main degradation enzyme, reached 96.1 U·L-1. Furthermore, from the UV-Vis spectrum of CR, the absorption peak at 495 nm vanished and new absorption peaks appeared in the ultraviolet region after 96 h and 192 h of decolorization. Benzidine is initially produced in the degradation process. It was isolated by HPLC and its identity was confirmed by FT-IR, mass spectrometry and NMR. The benzidine was itself degraded gradually over time.