| 1株海洋异养硝化-好氧反硝化菌的分离鉴定及其脱氮特性 |
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| 引用本文： | 孙庆花,于德爽,张培玉,林学政,李津.1株海洋异养硝化-好氧反硝化菌的分离鉴定及其脱氮特性[J].环境科学,2016,37(2):647-654. |
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| 作者姓名： | 孙庆花 于德爽 张培玉 林学政 李津 |
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| 作者单位： | 青岛大学环境科学与工程学院, 青岛 266071;青岛大学环境科学与工程学院, 青岛 266071;青岛大学环境科学与工程学院, 青岛 266071;国家海洋局第一海洋研究所, 国家海洋局海洋生物活性物质重点实验室, 青岛 266061;青岛大学环境科学与工程学院, 青岛 266071 |
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| 基金项目： | 国家自然科学基金项目(51278258;51478229);山东省自然科学基金项目(BS2015HZ007);山东省高等学校科技计划项目(J15LC61);青岛市应用基础研究项目(13-1-4-203-jch) |
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| 摘 要： | 以海水为基质,采用传统的微生物分离纯化方法,从海底沉积物中分离筛选得到1株耐盐异养硝化-好氧反硝化细菌y5,经形态、生理生化特性以及16S rRNA基因序列分析,鉴定该菌为克雷伯氏菌(Klebsiella sp.).对其脱氮特性及影响因素进行了研究,结果表明,菌株y5的最佳碳源为柠檬酸三钠,最适p H值为7.0,最适C/N为17.菌株均能以NH4Cl、Na NO2和KNO3为唯一氮源进行反应,36 h的去除率分别为77.07%、64.14%和100%.3种氮源共存时,36 h的去除率达到100%.表明菌株y5在高盐废水中具有独立高效的异养硝化和好氧反硝化作用.
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| 关 键 词： | 异养硝化 好氧反硝化 海洋环境 克雷伯氏菌 混合系统 脱氮特性 |
| 收稿时间： | 2015/7/13 0:00:00 |
| 修稿时间： | 2015/9/16 0:00:00 |
Identification and Nitrogen Removal Characteristics of a Heterotrophic Nitrification-Aerobic Denitrification Strain Isolated from Marine Environment |
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| SUN Qing-hu,YU De-shuang,ZHANG Pei-yu,LIN Xue-zheng and LI Jin.Identification and Nitrogen Removal Characteristics of a Heterotrophic Nitrification-Aerobic Denitrification Strain Isolated from Marine Environment[J].Chinese Journal of Environmental Science,2016,37(2):647-654. |
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| Authors: | SUN Qing-hu YU De-shuang ZHANG Pei-yu LIN Xue-zheng and LI Jin |
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| Institution: | School of Environmental Science and Engineering, Qingdao University, Qingdao 266071, China;School of Environmental Science and Engineering, Qingdao University, Qingdao 266071, China;School of Environmental Science and Engineering, Qingdao University, Qingdao 266071, China;Key Laboratory of Marine Bioactive Substances, First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China;School of Environmental Science and Engineering, Qingdao University, Qingdao 266071, China |
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| Abstract: | A heterotrophic nitrification-aerobic denitrification strain named y5 was isolated from marine environment by traditional microbial isolation method using seawater as medium. It was identified as Klebsiella sp. based on the morphological, physiological and 16S rRNA sequence analysis. The experiment results showed that the optimal carbon resource was sodium citrate; the optimal pH was 7.0; and the optimal C/N was 17. The strain could use NH4Cl, NaNO2 and KNO3 as sole nitrogen source, and the removal efficiencies were77.07%, 64.14% and 100% after 36 hours, respectively. The removal efficiency reached 100% after 36 hours in the coexistence of NH4Cl, NaNO2 and KNO3. The results showed that the strain y5 had independent and efficient heterotrophic nitrification and aerobic denitrification activities in high salt wastewater. |
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| Keywords: | heterotrophic nitrification aerobic denitrification marine environments Klebsiella sp heterotrophic nitrification-aerobic denitrification system characteristics of nitrogen removal |
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