间甲酚对杂色云芝(Coriolus versicolor)生长及产漆酶能力的影响

分别进行了间甲酚浓度为0mg·L-1,166 7mg·L-1和275mg·L-13种情况下的杂色云芝实罐发酵试验,定时测定了3种情况下菌丝干重(DC)、淀粉消耗、间甲酚降解和酶活性随时间的变化.在无间甲酚时,菌丝干重在188h达到最大值2600mg·L-1;在120h开始出现酶活,228h达到最大值82 15U·mL-1后,呈现一个稳定态势,不因衰亡期的到来而有较大幅度的变化.在加入166 7mg·L-1间甲酚时,菌丝干重在204h方达到最大值(995mg·L-1),且较无间甲酚时低得多(降低61 73%);在180h开始出现酶活,204h达到最大值(118 36U·L-1),并高出前者44 1%,此后也呈现一个稳定的态势.在加入间甲酚浓度为275mg·L-1时,菌丝干重在372h方达到最大值(320mg·L-1),但仅是无间甲酚的12 31%,间甲酚浓度为166 7mg·L-1时的32 16%;在264h开始出现酶活,396h达到最大值(112 8U·L-1),其酶活水平与间甲酚为166 7mg·L-1情况下的水平相当,这说明间甲酚可以诱导杂色云芝达到较高的产酶水平.研究发现在上述条件下杂色云芝生长的时间统计模型3种情况下的动力学模型可以用Logistic方程来模拟,即间甲酚的加入,并不改变其生长动力学模型;杂色云芝产漆酶的动力学模型在3种情况下可以分别用一级反应动力学方程描述;3种情况下,淀粉消耗均可以用Logistic模型来描述,从3个方

Effect of m-cresol on the growth of coriolus versicolor and its laccase product ion

A systematic research of m-cresol biodegradation dynamics by Corio lus versicolor was made. The fermentation characters by C. versicolor in 30 L fermen tor was performed and DC(dried cell), starch, m-cresol consumption, and lac case activity was determined with an 12h interval in three cases of 0mg·L^-1, 166.7m g·L^-1 and 275mg·L^-1 m-cresol. In the absence of the m-cresol, the maximum densi ty of DC was 2600mg·L^-1 at 188h, the enzyme activity appeared at 120h, reached the maximum of 82.15U/mL at 228h and then gradually remained stably during the l ater contabescence periods. In the presence of 166.7mg·L^-1m-cres ol,, the maxim um DC was 995mg·L^-1 at 204h, which was much lower (reduced to 61.73% of nom-cresol) than that in the absence of m-cresol, but the enzyme activity appeare d at 180h, the maximum was 118.36U·mL^-1 at 204h, 44.1%higher than tha t in the abse nce of m-cresol, and then was stable without obvious variation in the late r sta ge. In the presence of 275mg·L^-1 m-cresol, the maximum DC was 32 0mg·L^-1 at 37 2h, which was 12.31% of the absence of m-cresol and 32.16% of the prese nce of 1 66.7mg·L^-1 m-cresol. The enzyme activity appeared at 264h, and the maximum one w as112.8U·mL^-1 at 396h, which was similar to that at the 166.7mg· L^-1 m-cresol co ncentration, indicating that m-cresol could reduce the higher productivity of C. versicolor for laccase. The time-order model of Coriolus versicolor grow th at t hree concentrations of m-cresol above was studied and the kinetics equation s of the growth model were simulated with one order Logistic Equation. Thus, the addi tion of m-cresol doesn't change the growth kinetics model of Coriolus ver sicolor . The kinetics of the starch consumption could be described by a Logistic model, respectively in the above three cases, which indicated that m-cresol coul d, in part, replace or inhibit the consumption of the starch. The degradation kinetic s of m-cresol by C. versicolor in the fermentative liquid was also disc ussed. At m-cresol concentration of 275mg·L^-1, its degradation appeared wit h an unstabl e and slow degradation inclination. The different equations also showed that the degradation kinetics of m-cresol by C. versicolor was different at var ious concentrations.