| 湖南耐寒桉树优良单株组培研究 |
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| 引用本文： | 饶红欣,夏晓敏,彭信海,蒋丽媛,彭映辉,李轩.湖南耐寒桉树优良单株组培研究[J].湖南环境生物职业技术学院学报,1996(2). |
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| 作者姓名： | 饶红欣 夏晓敏 彭信海 蒋丽媛 彭映辉 李轩 |
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| 作者单位： | 湖南省森林植物园，湖南省森林植物园，湖南省森林植物园，湖南省森林植物园，湖南省森林植物园，湖南省森林植物园 长沙 410116，长沙 410116，长沙 410116，长沙 410116，长沙 410116，长沙 410116 |
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| 摘 要： | 本研究以湖南省林业技术推广总站在湖南选育的17个耐寒桉树优良单株为试验材料,进行组培繁殖,有14个优良单株成功地诱导出了愈伤组织或芽。结果表明,以一年生扦插苗的当年生嫩枝作为外植体来源,污染率低且愈伤组织和芽的诱导率高;0.25％氯化汞消毒15—20分钟,可将污染率控制在70％以下,且对外植体的损害最小;按树最佳的诱导培养基为:1/2MS+BA_(1.0)+IBA_(0.25),最佳的继代培基为:MS+BA_(1.0)+KT_(1.0)+VB2_(10)。
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| 关 键 词： | 耐寒按树 优良单株 组培 |
STUDY ON THE TISSUE CULTURE OF SUPERIOR COLD RESISTANT INDIVIDUAL OF EUCALYPTUS CAMALDULENSIS |
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| Rao Hongxin Xia Xiaomin Peng Xinhai Jiang Liyuan Peng Yinghui Li Xuan.STUDY ON THE TISSUE CULTURE OF SUPERIOR COLD RESISTANT INDIVIDUAL OF EUCALYPTUS CAMALDULENSIS[J].JOurnal of Hunan Environment Biological Polytechnic,1996(2). |
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| Authors: | Rao Hongxin Xia Xiaomin Peng Xinhai Jiang Liyuan Peng Yinghui Li Xuan |
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| Abstract: | The calli and/or buds have been induced from fourteen kinds o f superior cold-resistant Eucalptus camaldulensis selected in Hunan. Ace ording to our researchs the contamination ratio of the explant from one- year -old rooting cutting is low and the induction ratio is high;the tis sue was sterilized in 0. 25% HgCU for 15 - 20 minutes the contamination r atio is no more than 70% and the toxic to explant is least. The most sui table induction medium to E. camaldulen-sisis 1/2MS + BA1.0 + IBA0.25 and the most suitable subpassage medium is MS + BA1.0.+KT1.0+VB210(mg/L). |
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| Keywords: | Cold -resistant Eucalyptus camaldulensis Superior individual Tissue culture |
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