1，4-二氯苯降解菌的分离及其降解特性研究

从某污水处理曝气池的活性污泥中分离出一株能够以1，4-二氯苯为唯一碳源和能源生长的菌株DEB-1，通过形态特征和生理生化试验初步鉴定为黄杆菌属（Flavobacterium sp.）。实验结果表明，该菌株最适降解温度为32℃、最适降解pH为7.8，24 h对100 mg/L的1，4-二氯苯的降解率达94.5%。菌株DEB-1的降解谱较广，对5种氯苯类物质具有较高的降解率。并进一步研究了DEB-1的1，4-二氯苯降解酶粗酶液的性质，其最适反应温度和pH分别为30℃和8.5。对处理含氯代芳香化合物的有机废水具有一定的意义。

Study on isolation and characterization of a dichlorobenzene-degrading bacterial strain

A bacterial strain named DEB-1 that degraded dichlorobenzene as sole carbon and energy sources was isolated from activated sludge of a wastewater treatment plant. It was identified as Flavobacteriurn sp.according to its morphology and biochemical properties. The results showed that the optimal degradation tempera-ture of DEB-1 was 32 ℃,the optimal degradation pH value was 7.8,the degradation rate reached to 94.5% with-in 24 hours when the concentration of dichlorobenzene was 100 mg/L. The degradation spectrum of strain DEB-1 was broad and it had higher degradation for five chlorobenzenes. Further study on the characterization of strain DEB-1 degrading crude enzyme showed that the optimal reaction temperature and pH was 30 ℃ and 8.5 respec-tively. It could be useful for treating chlorinated-aromatic-containing organic wastewater.