The in-vitro response of pollen germination and tube length to different types of acidity

Pollen germination and tube growth are among the most sensitive responses to atmospheric pollution. Both these are inhibited by the acidity of the growth medium. Pollen grains from two species (Pinus cembra L. and Sambucus nigra L.) were germinated in media over a range of pHs (5.0, 4.5, 4.0, 3.5, 3.0 and 2.5) and six types of acidity (H(2)SO(4), HNO(3), H(2)SO(4): HNO(3) in 1:1, 2:1, 3:1 and 5:1 ratio). Pollen of the Gymnosperm is shown to be more resistant to acidity in the medium. Sulphuric acid alone and the ratio 2:1 with nitric acid are demonstrated to be the more harmful for P. cembra and S. nigra, respectively. The latter species was sensitive to all mixtures, particularly in respect to germination percentage.