后续碳源强化ANAMMOX-MFC系统脱氮产电调控策略

考察添加后续碳源的厌氧氨氧化微生物燃料电池（ANAMMOX-MFC）高效脱氮产电运行的稳定性，采用高通量测序及KEGG功能酶预测对微生物群落结构及功能基因的变化进行表征。结果表明:系统可以连续稳定运行60 d，TN去除率稳定在96%以上，连续运行最大输出电压为800 mV左右。添加适当浓度的乙酸钠，可增高污泥中发酵菌Enterococcus、反硝化产电菌Pseudomonas、Thauera的相对丰度进而有效促进产电。但是过度水解酸化会抑制AnAOB活性。第68~104天，出水NH₄+-N浓度升高，TN去除率下降至90%左右，通过停加乙酸钠TN去除率恢复至95%以上。ANAMMOX-MFC系统出水NH₄+-N浓度可作为后续碳源停加的"调控信号"，使得脱氮产电系统稳定高效运行，为厌氧氨氧化与微生物燃料电池脱氮产电的技术结合提供了很好的理论依据，具有一定的工程指导意义。

CONTROL STRATEGY OF SUBSEQUENT CARBON SOURCE IN ANAMMOX-MFC SYSTEM FOR ENHANCEMENT NITROGEN REMOVAL AND POWER GENERATION

This article mainly investigated the stability of anammox microbial fuel cell (ANAMMOX-MFC) high-efficiency denitrification and electricity generation operation with the addition of subsequent carbon sources, and used high-throughput sequencing and KEGG functional enzymes to predict changes in microbial community structure and functional genes characterization. The results showed that the system could run continuously and get stably for 60 days, the total nitrogen removal rate was 96% above, and the maximum output voltage of continuous operation was about 800 mV. Adding a proper concentration of sodium acetate could increase the relative abundance of the fermenting bacteria Enterococcus, denitrifying electricity-producing bacteria Pseudomonas, and Thaurea in the sludge, thereby effectively promoting electricity production. However, excessive hydrolysis and acidification will inhibit AnAOB activity. From the 68th to the 104th day, the ammonia nitrogen concentration in the effluent increased, and the total nitrogen removal rate dropped to about 90%. The total nitrogen removal rate was restored to 95% above by stopping the sodium acetate. The NH₄+-N concentration in the effluent of the ANAMMOX-MFC system could be used as a "regulation signal" for the subsequent stop of the carbon source, so that the denitrification and electricity generation system could run stably and efficiently. It provides a combination of anaerobic ammonia oxidation and microbial fuel cell denitrification and electricity generation technology and has a good theoretical basis and certain engineering significance.