脉冲与直流电场对厌氧氨氧化脱氮效果的影响

脉冲电场目前主要运用于食品、医疗灭菌等领域，其被广泛认可的灭菌机制为电穿孔理论。基于此，在较低电压和频率下脉冲电场可改变细胞通透性的同时能保证细胞不被灭活。为了探究脉冲电场对厌氧氨氧化脱氮效果的影响，将不同类型的电场分别施加在反应器中，以进出水NH₄+-N、NO₂--N浓度及微生物细胞结构为检测指标，考察各条件下的脱氮效果。结果显示，施加脉冲电场组能有效维持厌氧氨氧化反应的稳定，TN去除率可达到80%以上，相较于施加直流电场组和空白电场组，TN去除率分别提高了60%和20%。同时，脉冲电场组的微生物结构较直流电场组和空白电场组更加完整，表明脉冲电场能有效促进厌氧氨氧化脱氮效果并维持反应系统稳定。

EFFECT OF PULSED ELECTRIC AND DIRECT CURRENT FIELD ON NITROGEN REMOVAL BY AN ANAMMOX REACTOR

Pulsed electric field is mainly used in food and medical sterilization, and its widely recognized sterilization mechanism is electroporation theory. Based on that, the pulsed electric field at low voltage and frequency can not only change the permeability of cells, but also ensure that the cells can not be inactivated. In order to explore the influence of pulsed electric field on the denitrification effect of ANAMMOX, different types of electric fields were applied in the constructed reactor respectively. The concentration of influent and effluent NH₄+-N and NO₂--N and the microbial cell structure were taken as the detection indexes to investigate the denitrification effect under various conditions. The results showed that the pulsed electric field group could effectively maintain the stability of the ANAMMOX reaction, and the TN removal rate reached more than 80%, which was 60% and 20% higher than the TN removal rate in the DC electric field group and the blank group respectively. At the same time, the microorganism structure of pulsed electric field group was more complete than that of the DC electric field group and blank group. The results showed that the pulsed electric field could effectively promote the denitrification effect of ANAMMOX and maintain the running stability of the reaction system.