A sampler for the aseptic collection of water samples in the sea

A sampler is described which can be used for the collection of microbiological, phytoplanktological, and chemical sea-water samples. The sampler consist of a polyvinylchloride (PVC) frame on which a sterile silicone-rubber container (500 ml) is assembled. The sampler is fitted with a couple of reversing thermometers. All materials used, including the hydrographic cable and the messenger, have been tested for toxicity. The container, actuated by the messenger, fills and then is closed by a stop-cock to prevent any contamination with upper-level sea water during hauling aboard. The reversing thermometers reverse only when the sample has been taken properly. Thus, the thermometers guarantee good functioning of the sampler and supply also information on the exact depth at which the sample was collected. The device can operate at any depth and several devices can be operated in one cast. During normal operation, contamination due to microorganisms attached to the frame and container is negligible.This work was performed under the association contract Euratom-CNEN.