苯并\[a\]芘对马氏珠母贝肝组织抗氧化酶活性的影响

为揭示不同浓度苯并a]芘(Ba]P)对海洋贝类的生态毒理效应,将马氏珠母贝(Pinctada martensi)暴露于不同浓度(1、4和8μg.L-1)Ba]P中,检测暴露3、7和10d后,Ba]P对马氏珠母贝肝组织抗氧化酶(超氧化物歧化酶SOD、谷胱甘肽硫转移酶GST和过氧化氢酶CAT)活性的影响。结果表明:暴露时间3d时,随着Ba]P浓度的增加,SOD活性无明显变化,GST的活性被激活,在7d和10d受到抑制,GST活性表现为抑制,并表现出一定的剂量-效应关系,而CAT的活性在染毒后第7天受到激活;暴露时间10d时,SOD活性增加,GST活性和CAT活性均受到抑制。Ba]P暴露时间相同,GST活性和CAT活性变化趋势基本相似。Ba]P浓度相同(1和4μg.L-1)时,随着暴露时间的延长,SOD活性无明显变化,GST的活性受到抑制,CAT的活性表现为先激活后抑制的趋势。另外,相对于SOD活性,GST和CAT的活性变化可以更好地反映Ba]P对马氏珠母贝胁迫的毒性效应。

Effects of B\[a\]P on Activity of Antioxidant Enzyme in Liver of Pinctada martensi

In order to reveal the ecotoxicological effects of Ba]P on marine mollusks,influence of concentration of Ba]P on activity of superoxide dismutase(SOD),glutathione S-transferase(GST)and catalase(CAT)in liver of Pinctada martensi were studied.Specifically,Pinctada martensi was exposed to Ba]P for 3,7,10 and 13 days,respectively,at three different Ba]P concentrations(1,4 and 8 μg·L-1).The results show that no significant influence of concentration of Ba]P on the activity of SOD was observed at 3rd day.GST activity was inhibited after exposure of 7th day and 10th day in Ba]P.There exists a significant dose-effect relationship between GST activity and concentration of Ba]P.CAT activity was induced at the exposure of 7th day of Ba]P.Compared to the control group,SOD activity increased while GST and CAT activity decreased after exposure of 10th day in Ba]P.Similar trends between exposure time at the same exposure concentration of Ba]P(1 and 4 μg·L-1)and the activity of SOD and GST were observed.However,CAT activity was induced and then depressed by Ba]P with increasing of exposure time.Compared with the activity of SOD,the variations of activity of GST and CAT enzyme could better reflect the ecotoxicological effects of Ba]P on Pinctada martensi in liver.