蒽醌类化合物对大型蚤(Daphnia magna)的急性光致毒性研究

通过测定模拟光照和无光照条件下20种蒽醌类化合物对大型(Daphnia magna)的48h半数活动抑制浓度(EC50), 研究了该类化合物的光致毒性．比较了两种助溶剂(二甲基亚砜和丙酮)对这些化合物光致毒性的影响,初步讨论了光致毒性的机理．结果表明,所研究的助溶剂不影响这些化合物对大型潘的光致毒性．蒽醌类化合物因其取代基的不同(-NH2、-OH、-Cl、-Br、-NO2)、取代基位置的不同和取代基数目的不同,而表现出对大型潘光致毒性的不同．最低未占据分子轨道能(ELUMO)和最高占据分子轨道能(EHOMO)之间的能级差,可用于初步判断蒽醌类化合物 溞大型的潜在光致毒性．

Acute Photoinduced Toxicity of Anthraquinones to the Crustacean Daphnia magna

In order to study the acute photoinduced toxicity of anthraquinones, the 48 h acute immobilization toxicity of 20 anthraquinones to Daphnia magna was determined, under two different light/dark cycles, 14 h (light):10 h (dark), and sole dark. Daylight fluorescent lamp was employed to simulate sunlight. The effects of two solubilizing agents, DMSO and acetone, on the photoinduced toxicity were compared and the mechanism of the photoinduced toxicity was also discussed. The two co-solvents did not affect the photoinduced toxicity of the compounds. The photoinduced toxicity of the substituted anthraquinones depended on the number, position and electron-donating ability of the substituent groups to the parent structure of anthraquinone. The gap between the energy of the highest occupied molecular orbital and the lowest unoccupied molecular orbital was an indicator for the potential photoinduced toxicity of anthraquinones to Daphnia magna.