Spatial and seasonal variations in bacterial communities of the Yellow Sea by T-RFLP analysis |
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Authors: | Hongyuan Wang Xiaolu Jiang Ya He and Huashi Guan |
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Institution: | (1) School of Medicine and Pharmacy, Ocean University of China, Qingdao, 266003, China;(2) Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Science, Beijing, 100081, China;(3) School of Environment, Beijing Normal University, Beijing, 100875, China |
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Abstract: | Four typical coastal sites (rocky shore, sandy shore, mud flat shore, and artificial harbor) at the Yellow Sea were chosen
to investigate the spatial and seasonal variations in bacterial communities. This was accomplished by using terminal restriction
fragment length polymorphism (T-RFLP) analysis of PCR amplified 16S rDNA fragments. Two kinds of tetrameric restriction enzymes,
HhaI and MspI, were used in the experiment to depict the bacterial community diversity in different marine environments. It was found
that the community compositions digested by the two enzymes separately were different. However, the results of bacterial community
diversity derived from them were similar. The MDA analysis results of T-RFLP profiles coming from HhaI and MspI both exhibited a significant seasonal community shift for bacteria and a relatively low spatial variation among the four
locations. With HhaI as the sample, the pair wise T-tests also revealed that variations were minor between each pair of marine environments, with R ranging from 0.198 to 0.349. However, the bacterial community structure in the mud flat site depicted a larger difference
than each of the other three sites (R ranging from 0.282 to 0.349). |
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Keywords: | terminal restriction fragment length polymorphism (T-RFLP) bacterial community structure marine microbial diversity HhaI MspI |
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