| Ni2+金属结合肽的筛选和序列分析 |
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| 引用本文： | 徐柳,谢明德,佟鑫,马春燕,茆灿泉.Ni2+金属结合肽的筛选和序列分析[J].应用与环境生物学报,2006,12(3):379-380. |
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| 作者姓名： | 徐柳 谢明德 佟鑫 马春燕 茆灿泉 |
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| 作者单位： | 西南交通大学生物工程学院,成都,610031 |
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| 基金项目： | 国家自然科学基金;西南交通大学校科研和教改项目 |
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| 摘 要： | 利用噬菌体随机十二肽库对金属N i2 进行了结合肽筛选,经5轮生物淘洗、噬菌体扩增和DNA测序,获得8条多肽序列,分析发现其中富含组氨酸(4~6个/肽).结果表明,组氨酸的存在对蛋白质和金属N i2 的结合起着关键作用.图1表1参11
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| 关 键 词： | Ni2 噬菌体随机肽库 结合肽 筛选 |
| 收稿时间： | 2005-03-03 |
| 修稿时间： | 2005-06-30 |
Selection and Sequence Analyses of High Affinity Binding Peptides for Ni2+ |
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| XU Liu,XIE Mingde,TONG Xin,MA Chunyan,MAO Canquan.Selection and Sequence Analyses of High Affinity Binding Peptides for Ni2+[J].Chinese Journal of Applied and Environmental Biology,2006,12(3):379-380. |
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| Authors: | XU Liu XIE Mingde TONG Xin MA Chunyan MAO Canquan |
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| Institution: | College of Bioengineering, Southwest Jiaotong University, Chengdu 610031, China |
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| Abstract: | Phage random 12-peptide library was used for selecting peptide binding for Ni~(2 ).After five rounds of biopanning,phage amplification and DNA sequencing,a group of 12-peptides were obtained from the library.The sequences of these DNA coding peptides were sequenced and identified,containing four to six histidine residues.The results showed histidine residues were very important in the binding peptides for Ni~(2 ).Fig 1,Tab 1,Ref 11 |
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| Keywords: | Ni~(2 ) phage random peptide library binding peptide biopanning |
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