表面活性剂对Bacillus pumilus strain Bap9降解苯并[a]芘的影响

研究了非离子型表面活性剂Triton X-100（TX-100）和Tween80（TW-80）对苯并a]芘的增溶特性及对苯并a]芘高效降解菌Bacillus pumilus strain Bap9生长的影响,结果表明,2种表面活性剂对苯并a]芘均有良好的增溶效果,均能作为碳源和能源被菌株Bap9所利用,TX-100增溶能力和增殖能力相对更强;不同浓度的TX-100对菌株降解苯并a]芘的影响不同,当浓度为1 000 mg/L时,对降解的促进作用最强,可将苯并a]芘降解率提高20.8%;在苯并a]芘降解过程中,TX-100亦能作为碳源被菌株Bap9利用,不产生二次污染,因此可用于苯并a]芘污染环境的生物修复。

Effect of surfactants on biodegradation of benzo [a ]pyrene by Bacillus pumilus strain Bap9

The impacts of two nonionic surfactants（TX-100 and TW-80） on the solubility of benzoa]pyrene（BaP） and biomass production of Bacillus pumilus strain Bap9 were studied.Results show that TX-100 and TW-80 were not toxic to the BaP-degrading strain Bap9,and both of them could greatly increase the solubility of BaP when their concentration were above the critical micelle concentration（CMC）.TX-100 is more effective than TW-80 in increasing solubility of BaP and proliferation of strain Bap9.The effect of degradation was best when the concentration of TX-100 reached 1000 mg/L,which increased 20.8% of the degradation rate.During the biodegradation of BaP,TX-100 could be utilized by the strain Bap9 as carbon source and would not produce any secondary pollution.As a result,TX-100 has considerable potential for the bioremediation of BaP in polluted soils and water.