处理含酚废水曝气生物流化床中丝状菌种群结构的解析

应用曝气生物流化床反应器(ABFT)处理含酚废水，随着进水ρ(苯酚)由100 mg/L提高到400 mg/L，苯酚的去除率稳定在100%左右，且苯酚的去除主要发生在4个反应池中的第一池. 利用扫描电镜(SEM)、荧光原位杂交(FISH)和变性梯度凝胶电泳(DGGE)技术对反应器内微生物种群进行分析. 扫描电镜结果表明，随着反应器进水ρ(苯酚)的提高，填料生物膜微生物逐渐演变为以丝状菌为主. 针对SSU rRNA的荧光原位杂交结果表明，反应器内丝状微生物主要为真菌. 分别对细菌和真菌的16S rDNA和18S rDNA 进行PCR-DGGE分析，结果表明，反应器内主要的苯酚降解菌为克氏地霉(Geotrichum klebahnii)，同时存在发硫菌等丝状细菌. 聚类及多样性分析表明，苯酚对微生物种群表现出很强的选择性. 

Analysis of the Structure of Filamentous Microbial Groups from Aerated Biological Fluidized Tank Treating Phenolic Wastewater

An aerated biological fluidized tank (ABFT) was used to treat phenolic wastewater with favorable treatment efficiency. As the influent phenol concentration increased from 100 mg/L to 400 mg/L, the phenol removal rate remained around 100%, and stable phenol removal was obtained at the first of four tanks. The microbial community structure and biodiversity in the ABFT reactor were examined using methods such as scanning electron microscopy (SEM), fluorescence in situ hybridization (FISH) and denaturing gradient gel electrophoresis (DGGE). The results of SEM showed that with the increase of influent phenol concentration, filamentous microorganisms gradually became the dominant species in the membrane of the ABFT stuffing. The FISH results targeting SSU rRNA revealed that most of the filamentous microorganisms in the ABFT reactor were fungi, while the PCR-DGGE results for bacterial and fungal 16S and 18S rDNA indicated that the filamentous fungi mainly belonged to Geotrichum klebahnii, although there were also some filamentous bacteria, such as Thiothrix sp. and actinobacterium. Cluster analysis and diversity analysis showed that phenol had strong selectivity on the microbial community.