改性煤矸石对上覆水磷及底泥磷代谢关键基因的影响

将热活化煤矸石和镧改性煤矸石应用于封闭水体除磷固磷试验，采用16SrRNA高通量测序技术分析底泥微生态群落结构、聚磷细菌和磷代谢功能基因的变化.结果表明：镧改性煤矸石对上覆水TP的去除能力最高，稳定期上覆水TP浓度为0.023~0.028mg/L，较对照组低83.5%以上，热活化煤矸石对上覆水TP的去除能力较差，稳定期上覆水TP浓度为0.15mg/L左右，略低于对照组.热活化煤矸石和镧改性煤矸石均提高了底泥中微生物多样性，变形菌门（Proteobacteria）和绿弯菌门（Chloroflexi）为底泥优势菌种.不同处理组底泥中聚磷细菌为Tetrasphaera和Candidatus_Accumulibacter，镧改性煤矸石显著降低了底泥中聚磷细菌的相对丰度.热活化煤矸石和镧改性煤矸石对多聚磷酸盐激酶（PPK）影响不大，但对外切聚磷酸酶（PPX）的抑制较大，热活化煤矸石抑制最大.

Effect of modified coal gangue on Phosphorus in overlying water and key genes of phosphorus metabolism in sediment

Thermal activated coal gangue and lanthanum modified coal gangue were used in the experiments of phosphorus removal and fixation in closed water. 16SrRNA high-throughput sequencing technology was used to analyze the changes of microbial community structure, phosphorus accumulating microorganisms and phosphorus metabolism function genes in sediment. Lanthanum modified coal gangue had the best removal ability of total phosphorus (TP) from the overlying water. The concentration of TP in the overlying water was 0.023~0.028 mg/L in the stable period, which was 83.5% lower than that in the control group. The removal ability of TP from the overlying water by thermal activated coal gangue was poor and its concentration in the overlying water in the stable period was about 0.15mg/L, which was slightly lower than that in the control group. Thermal activated coal gangue and lanthanum modified coal gangue both improved the microbial diversity in the sediment.Proteobacteria and Chloroflexa were the dominant bacteria in the sediment. The phosphorus accumulating organisms in the sediment in different treatment groups were Tetraspeaera and Candidatus_Accumulibacter, and their relative abundance was significantly reduced by lanthanum modified caol gangue.these two modified coal gangue both had little effect on polyphosphate kinase (PPK), but an obvious inhibition on polyphosphate exonuclease (PPX) especially for thermal activated coal gangue.