水中杀菌剂叶青双及其降解物的反相液相色谱测定

本文用反相液相色谱法测定水中叶青双及其降解物2-氨基5-巯基-1,3,4-噻二唑。平均回收率分别为91％和94％;对水样的最低检测浓度分别为2ppb和0.8ppb;叶青双与2-氨基-5-巯基-1,3,4-噻二唑的分离度为2.5。本法在柱效和灵敏度上比硅胶柱液相色谱法有明显的提高。

RP-HPLC DETERMINATION OF GERMICIDE MBAMT AND ITS DECOMPOSITION PRODUCT IN AQUATIC ENVIRONMENT

This paper presented a method of reversed phase high performance liquid chromatography to determine the germicide N, N-methylene-bi-(2-amino-5-mercapto-1,3,4-thidiazole) (MBAMT) and it's decomposition product 2-amino-5-mercapto-l,3,4-thidia-zole (AMT). The sample was enriched, extracted and purged through Amberlite XAD-7 or XAD-8.The chromatography conditions were as follows:column:Lichrosorb RP-18;mobile phase: methanol-acetonitrile-water (23:22:55), pH-3.6. Detection at 313 nm with ultraviolet detector. Quantitative determination with external standard method. Recoveries of MBAMT and AMT were 90% and 94% respectively. The detected limits of concentration for water sample were 2 ppb for MBAMT and 0.8 ppb for AMT. The resolution between the peaks of MBAMT and AMT was 2.5. The column efficiency and detected sensitivity of this method were significantly better in comparision with normal phase chromatography.