Protein kinase C and ion transport in the posterior gills of the Chinese crabEriocheir sinensis |
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Authors: | V Asselbourg G Trausch R Gilles P Devos |
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Institution: | (1) Department of Biology, Facultés Universitaires Notre-Dame de la Paix, 61 rue de Bruxelles, B-5000 Namur, Belgium;(2) Laboratory of Animal Physiology, University of Liège, 22 quai Van Beneden, B-4020 Liège, Belgium |
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Abstract: | The possible involvement of protein kinase C in control of ion transport was investigated on a preparation of isolated, perfused posterior gills of the Chinese crabsEriocheir sinensis (collected in 1989 from lakes near Emden, northern Germany) acclimated to fresh water. 1-oleyl-2-acetyl-sn-glycerol (OAG) and phorbol 12-myristate 13-acetate (PMA), two activators of protein kinase C, when added to the perfusion saline, induced depolarisation of the transepithelial potential difference (PD) and an increase in transepithelial Na+ influx. The observed increase was proportional to OAG concentration up to 250µM, with a 2.5× accelerated Na+ influx. OAG and PMA remained without effect on Cl– fluxes. The observed effects were in agreement with an activation, via protein kinase C, of the Na+/K+ ATPase located on the serosal side of the epithelium. |
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