Biohydrogen using leachate from an industrial waste landfill as inoculum |
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Authors: | Hisanori Watanabe Hidekichi Yoshino |
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Institution: | (1) Kanagawa Environmental Research Center, 1-3-39 Shinomiya, Hiratsuka Kanagawa, 254-0014, Japan;(2) Institute of Environment and Resource Systems, 11-23 Moegino, Aobaku, Yokohama 227-0044, Japan |
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Abstract: | Since hydrogen is a renewable energy source, biohydrogen has been researched in recent years. However, data on hydrogen fermentation
by a leachate from a waste landfill as inoculum are scarce. We investigated hydrogen production using a leachate from an industrial
waste landfill in Kanagawa Prefecture. The results showed no methane gas production, and the leachate was a suitable inoculum
for hydrogen fermentation. The maximum H2 yield was 2.67 mol of H2 per mol of carbohydrate added, obtained at 30°C and an initial pH of 7. The acetate and butyrate production was significant
when the H2 yield was higher. Oxidation–reduction potential analysis of the culture suggested that hydrogen-producing bacteria in the
leachate were facultative anaerobic. Scanning electron microscope observations revealed that the hydrogen-producing bacteria
comprised bacilli about 2 μm in length. |
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Keywords: | |
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