改进的PVA-硫酸盐法固定蛋白核小球藻除磷研究

首次报道采用改进后的PVA-硫酸盐固定化方法,包埋固定蛋白核小球藻.该方法克服了现有的几种PVA固定化包埋过程的缺点.其操作过程简单,形成的凝胶小球不黏连,透光性好,对生物毒性小,机械强度高.经固定的蛋白核小球藻在光照条件下对磷的去除率达95%,3个周期后,磷去除率仍可达到75%以上,凝胶小球强度没有明显降低.实验证明,光照是影响固定化蛋白核小球藻除磷的主要因素,光暗比12:12与24h全光照的两组对磷的最高去除率没有明显差别,但光暗比12:12的一组去除率有波动.透射电镜观察结果表明,PVA与细胞壁紧密粘贴,蛋白核小球藻生长没有停止.

Studies on phosphorus removal by Chlorella pyrenoidosa with modified PVA-sulfate immobilization method

In this study it is reported for the first time that the method of modified PVA-sulfate immobilization has been adopted with entrapping of immobilized Chlorella pyrenoidosa. The operation of this method is simple, and the gel beads formed are not adhesive, with less biological toxicity, higher mechanical strength and good transparency. Some disadvantages of the existing PVA immobilization entrapping are avoided. Phosphorus removal rate can reach 95% with immobilized Chlorella pyrenoidosa under illumination. After 3 circles, the removal rate is still more than 75% and the strength of the gel beads does not decrease evidently. The two illumination periods, light:dark=24:0 and 12:12, show no significant difference in the maximum phosphorus removal rate. However, there is a fluctuation of the removal rate in 12 hours?light period. Transmission electron microscope (TEM) observation result demonstrates that PVA adheres closely to the cell wall, and the growth of Chlorella pyrenoidosa continues.